Histogenesis of neuroimmune in the mouse retina

LI Rui-ping XU Gao-lei SUI Yi-zheng WANG Lai FAN Wen-juan DENG Jin-bo*

doi:10.16098/j.issn.0529-1356.2018.05.001

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Acta Anatomica Sinica ›› 2018, Vol. 49 ›› Issue (5) : 561-570. DOI: 10.16098/j.issn.0529-1356.2018.05.001

Neurobiology

Histogenesis of neuroimmune in the mouse retina

LI Rui-ping¹ XU Gao-lei² SUI Yi-zheng¹ WANG Lai¹ FAN Wen-juan¹ DENG Jin-bo ^1*

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Abstract

Objective To investigate the histogenesis of neuroimmune system, such as microglia and blood retinal barrier (BRB), in the mouse retina. Methods Immunofluorescent staining, DiI diotistic assays, gelatin-ink perfusion and transmission electron microscopy (TEM) were used to visualize the structures of retinal microglia and BRB of Kunming mice at various ages (n=5-10). Results At as early as embryonic day 10(E10), the microglia distributed over retina evenly. With development, the microglia changed from an amoeba-like shape to a star-like shape with many processes. The number of microglia increased after the birth and reached their plateau at postnatal day 5 (P5), then felled down after P30. After birth, the retinal vasculature developed from the optic disk, and grew out to entire retina at P10. As age increasing, the volume density of vasculature was declined. The blood retinal barriers appeared as early as at P0. At P30, they became mature, consisting of endothelial cell, basal membrane, pericyte and the terminal feet of astrocytes. Conclusion As retinal development, microglia become more and more mature, and their number changed with parabolic model. At P30, the BRB have been developed maturely in the structures with endothelial cell, basal membrane, pericyte and the terminal feet of astrocytes. The microglia and BRB are involved in anti-infection and resistance to various pathogens.

Key words

Blood retinal barrier / Microglia / Retina / Neuroimmunology / DiI diotistic assays / Gelatin-ink perfusion / Transmission electron microscopy / Mouse

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LI Rui-ping XU Gao-lei SUI Yi-zheng WANG Lai FAN Wen-juan DENG Jin-bo. Histogenesis of neuroimmune in the mouse retina[J]. Acta Anatomica Sinica. 2018, 49(5): 561-570 https://doi.org/10.16098/j.issn.0529-1356.2018.05.001

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