Expression and age-related changes of centromere A in rat heart

FENG Ying YIN Guo-tian LI Ai-rong* GUO Zhi-kun*

doi:10.16098/j.issn.0529-1356.2018.04.016

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Acta Anatomica Sinica ›› 2018, Vol. 49 ›› Issue (4) : 512-517. DOI: 10.16098/j.issn.0529-1356.2018.04.016

Histology,Embryology and Developmental Biology

Expression and age-related changes of centromere A in rat heart

FENG Ying YIN Guo-tian LI Ai-rong* GUO Zhi-kun*

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Abstract

Objective To study the expression and age-related changes of centromere protein A(CENP-A) in the rat heart. Methods Ten rats in each group were collected from postnatal 1 day, 1 week old, 2 weeks old, 3 weeks old, 4 weeks old, 3 months old, 6 months old. The expression and age-related changes of CENP-A in the rat heart were observed by the immunohistochemistry, immunofluorescence, Western blotting, and Real-time PCR techniques. The CENP-A proteins of seven groups were quantitated by SPSS 20.0 statistical software. Results CENP-A was mainly expressed in the vascular wall, epicardium and epicardial tissue and in a very few cardiomyocytes. The CENP-A in vascular wall was of stable expression with age. But the expression of CENP-A in the epicardium and subepicardial tissues was gradually reduced, which showed that postnatal 1 day was the most and 6 months old are the least. CENP-A in the vascular wall was mainly expressed in the smooth muscle cells, and was basically unchanged with ages.A small number of cardiac myocytes near the subepicardium also express CENP-A, and decreased with age. Conclusion The expression of CENP-A in the rat heart gradually decreased with aging, but in the vascular wall remained stable expression.

Key words

Centromere protein-A / Myocardial tissue / Epicardium / Heart / Immunohistochemistry / Immunofluorescence / Rat

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FENG Ying YIN Guo-tian LI Ai-rong GUO Zhi-kun. Expression and age-related changes of centromere A in rat heart[J]. Acta Anatomica Sinica. 2018, 49(4): 512-517 https://doi.org/10.16098/j.issn.0529-1356.2018.04.016

References

［1］ Liang QJ. Centromeric and kinetochore ［J］. Bulletin of Biology,2012,47(4):1-4.(in Chinese)

梁前进.着丝粒和动粒［J］.生物学通报,2012,47(4):1-4.

［2］ Li YM,Zhu MH.Advances in biological functions of centromere protein A ［J］. Chinese Journal of Pathology,2006,35(12):750-751. (in Chinese)

李咏梅,朱明华.着丝粒蛋白A的生物学功能研究进展［J］.中华病理学杂志,2006,35(12):750-751.

［3］ Athwal RK, Walkiewicz MP, Baek S, et al. CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells［J］.Epigenetics Chromatin, 2015, 8（1）:2.

［4］ Zhang W, Mao JH, Zhu W, et al. Centromere and kinetochore gene misexpression predicts cancer patient survival and response to radiotherapy and chemotherapy［J］.Nat Commun, 2016,7:12619.

［5］ Li YM,Liu XH, Cao XZh，et al. Expression of centromere protein A in primary hepatocellular carcinoma ［J］. Chinese Journal of Pathology,2007,36(3):175-178. (in Chinese)

李咏梅,刘晓红,曹晓哲，等.着丝粒蛋白A在原发性肝癌中的表达［J］.中华病理学杂志,2007,36(3):175-178.

［6］ Li Y, Zhu Z, Zhang S, et al. ShRNA-targeted centromere protein A inhibits hepatocellular carcinoma growth［J］. PLoS One, 2011,6(3):e17794.

［7］ Maehara K, Takahashi K. CENP-A reduction induces a p53-dependent cellular senescence response to protect cells from executing defective mitoses［J］. Mol Cell Biol, 2010, 3(9):2090-2104.

［8］ McGregor M, Hariharan N, Joyo AY, et al. CENP-A is essential for cardiac progenitor cell proliferation［J］. Cell Cycle, 2014, 13(5):739-748.

［9］ Howman EV, Fowler KJ, Newson AJ, et al. Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice［J］. Proc Natl Acad Sci USA, 2000,97(3):1148-1153.

［10］ Park KC, Lee M, Jeon Y, et al. Skin-specific deletion of mis18α impedes proliferation and stratification of epidermal keratinocytes［J］. Invest Dermator, 2017,137(2):414-421.

［11］ Chen JJ, Zhao Q. Myocardial regeneration——from parenchyma to interstitial ［J］. International Journal of Cardiovascular Disease, 2013,40(5):285-287. (in Chinese)

陈俊佶, 赵强. 心肌再生——从实质到间质［J］. 国际心血管病杂志, 2013,40(5):285-287.

［12］ Bergmann O, Bhardwaj RD, Bernard S. Evidence for cardiomyocyte renewal in humans［J］. Science, 2009,324(5923):98-102.

［13］ Han C, Nie Y, Lian H, et al. Acute inflammation stimulates a regenerative response in the neonatal mouse heart［J］. Cell Res, 2015,25(10):1137-1151.