Epithelial-mesenchymal transition during the development of pulmonary endoderm-associated second heart field in mouse embryos

SHI Liang LI Hui-chao JING Ya* XIE Jian-shan CHEN Hao CAI Yu-jin YANG Yan-ping

doi:10.16098/j.issn.0529-1356.2018.04.011

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Acta Anatomica Sinica ›› 2018, Vol. 49 ›› Issue (4) : 480-485. DOI: 10.16098/j.issn.0529-1356.2018.04.011

Histology,Embryology and Developmental Biology

Epithelial-mesenchymal transition during the development of pulmonary endoderm-associated second heart field in mouse embryos

SHI Liang¹ LI Hui-chao ^1,2 JING Ya ^1* XIE Jian-shan¹ CHEN Hao^1,3 CAI Yu-jin¹YANG Yan-ping¹

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Abstract

Objective To explore the relationship between epithelial-mesenchymal transition (EMT) andthe development of pulmonary endoderm-associated second heart field in mouse embryos. Methods The transforming growth factor-β(TGF-β) 2 and TGF-β3 expressions in mouse embryonic hearts from embryonic day (ED) 9 to ED14 were detected by Western blotting. Both the immunohistochemical and immunofluorescent staining method were used to reveal forkhead box A2 (Foxa2), islet-1 (ISL-1), laminin (LN) and E-cadherin (E-cad) expressions in serial sections of 35 mouse embryos from ED10 to ED12. Results From ED10 to ED12, both TGF-β2 and TGF-β3 expressed in mouse embryonic heart. In the pulmonary endoderm-associated second heart field development between ED10 and ED12, there was no clear boundary between some ISL-1 positive pulmonary endoderm and surrounding ISL-1 positive second heart field mesenchym which seemed to be intermingled. At the same time, the basement membrane underlying the pulmonary endoderm became interrupted or blurred. Especially at ED11 and ED12, some pulmonary endoderm or its solid cord cells lost Foxa2 expression and showed strongly ISL-1 positive, accompanied by their basement membrane interrupting or missing, E-cad expression decreasing, obvious intercellular space emerging and detachment from endoderm, which suggested the loss of apico-basal polarity. Conclusion Epithelial-mesenchymal transition is involved in the development of pulmonary endoderm-associated second heart field of mouse embryos.

Key words

Epithelial-mesenchymal transition

/ Pulmonary endoderm / Second heart field / Transforming growth factor-β / Immunofluorescence / Western blotting / Mouse

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SHI Liang LI Hui-chao JING Ya XIE Jian-shan CHEN Hao CAI Yu-jin YANG Yan-ping. Epithelial-mesenchymal transition during the development of pulmonary endoderm-associated second heart field in mouse embryos[J]. Acta Anatomica Sinica. 2018, 49(4): 480-485 https://doi.org/10.16098/j.issn.0529-1356.2018.04.011

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