Effects of intervention of brain lipid binding protein expression on differentiation of neural stem cells

YU Ting-ting LI Hao-ming JIN Guo-hua* HAN Xiao QIN Jian-bing

doi:10.16098/j.issn.0529-1356.2018.04.002

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Acta Anatomica Sinica ›› 2018, Vol. 49 ›› Issue (4) : 424-430. DOI: 10.16098/j.issn.0529-1356.2018.04.002

Neurobiology

Effects of intervention of brain lipid binding protein expression on differentiation of neural stem cells

YU Ting-ting LI Hao-ming JIN Guo-hua* HAN Xiao QIN Jian-bing

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Abstract

Objective To explore the effect of BLBP on differentiation of hippocampal neural stem cells (NSCs) into neurons in vitro. Methods BLBP overexpression adenovirus and BLBP RNA interference lentiviral vector were built. NSCs were obtained from rat hippocampus by serum-free culturing and immunofluorescence was used to identify NSCs. The NSCs were assigned to Ad-NC group, Ad-BLBP group, LV-NC-RNAi group and LV-BLBP-RNAi group. On the 4th day after infection, BLBP mRNA and protein expression in the NSCs were detected by Real-time PCR and Western blotting, and the neuronal class Ⅲ β-tubulin (Tuj1) positive cells were detected by immunofluorescence. Results NSCs in Ad-BLBP group expressed a high level of BLBP and there were only fewer Tuj1 positive cells than the Ad-NC group; NSCs in LV-BLBP-RNAi group expressed a lower level of BLBP and there were more Tuj1 positive cells than the LV-NC-RNAi group. Conclusion Down regulation of BLBP expression can promote the differentiation of NSCs into neurons in vitro.

Key words

Brain lipid binding protein / Neural stem cells / Differentiation / Neurons / Western blotting / Rat

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YU Ting-ting LI Hao-ming JIN Guo-hua HAN Xiao QIN Jian-bing. Effects of intervention of brain lipid binding protein expression on differentiation of neural stem cells[J]. Acta Anatomica Sinica. 2018, 49(4): 424-430 https://doi.org/10.16098/j.issn.0529-1356.2018.04.002

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