Inhibitory effect of dihydromyricetin combined with etoposide on the choriocarcinoma cell JAR

WANG Kang XU Qian ZUO Yan-zhen LIU Lei LU Yan-jie LEI Yun-tao CUI Ying LI Yu-hong*

doi:10.16098/j.issn.0529-1356.2018.03.010

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Acta Anatomica Sinica ›› 2018, Vol. 49 ›› Issue (3) : 330-336. DOI: 10.16098/j.issn.0529-1356.2018.03.010

Cancer Biology

Inhibitory effect of dihydromyricetin combined with etoposide on the choriocarcinoma cell JAR

WANG Kang¹ XU Qian² ZUO Yan-zhen³ LIU Lei⁴ LU Yan-jie¹ LEI Yun-tao¹ CUI Ying1 LI Yu-hong^5*

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Abstract

Objective To investigate the inhibitory effect and related mechanism of dihydromyricetin(DMY) combined with etoposide(VP16) on the choriocarcinoma cell JAR.Methods JAR cells cultured in vitro were devided into control group, DMY group, VP16 group and DMY+VP16 group. The cell proliferation was measured by MTT assay. The apoptosis rate of each group was analyzed by annexin V-FITC/PI double dye flow cytometry. The cell viability was measured by the colony formation capability assay. The protein expression levels of Bcl-2, inhibitor apoptosis protein 2(c-IAP2) were detected by Western blotting analysis. Results The result of MTT assay showed that compared with the control group, the cell survival ratio of DMY group, VP16 group and DMY+VP16 group reduced at 24 hours and 48 hours.The result of double dye flow cytometry showed that the apoptosis rate of the experimental groups were higher than that of the control group, and the apoptosis rate of DMY+VP16 group was significantly higher than those treated with DMY or VP16 alone.The result of the colony formation capability assay showed that,for JAR cells,the number of clones in experimental groups were less than that in the control group, and the number of clones in DMY+ VP16 group was the least. For normal liver HL7702 cells, there was no significant difference between DMY group and the control group, and there was no significant difference between VP16 group and DMY+VP16.The result of Western blotting showed that the protein expression levels of Bcl-2 and c-IAP2 in experimental group decreased compared with those in the control group, and the decrease of he protein expression levels of Bcl-2 and c-IAP2 in DMY+VP16 group were the most significant. Conclusion Dihydromyricetin combined with etoposide enhanced the inhibitory effect on choriocarcinoma cell JAR, which can reduce the dosage of the chemotherapy drug VP16.The inhibitory effect of tumor cells may be related to the down-regulation of protein expression of Bcl-2, C-IAP2.

Key words

Dihydromyricetin / Etoposide / JAR cell / Western blotting / Human

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WANG Kang XU Qian ZUO Yan-zhen LIU Lei LU Yan-jie LEI Yun-tao CUI Ying LI Yu-hong. Inhibitory effect of dihydromyricetin combined with etoposide on the choriocarcinoma cell JAR[J]. Acta Anatomica Sinica. 2018, 49(3): 330-336 https://doi.org/10.16098/j.issn.0529-1356.2018.03.010

References

［1］Pang JL,Chen J,LI YY,et al.Effect of curcumin on invasion and adhesion of human choriocarcinoma JAR cell strains［J］.Maternal and Child Health Care of China,2012,27(3): 423-425.（in Chinese）

庞江琳,陈杰,李英勇,等.姜黄素对人类绒癌JAR细胞株侵袭粘附的影响［J］.中国妇幼保健,2012,27(3): 423-425.

［2］Zhang KY,Zuo XL,Li YH,et al.Effects of transforming growth factor-β1 on the invasiveness of the JEG-3,the protein expression of uPA,uPAR and PAI-1［J］.Maternal and Child Health Care of China, 2014,29(5):777-780.（in Chinese）

张孔雁,左欣鹭,李玉红,等.TGF-β1对绒毛膜癌JEG-3细胞侵袭能力及其uPA、uPAR、PAI-1蛋白表带的影响［J］.中国妇幼保健, 2014,29(5):777-780.

［3］Bromberg KD, Burgin AB, Osheroff N. A two-drug model for etoposide action against human topoisomerase Ⅱ alpha［J］. Journal Biol Chem, 2003, 278(9):7406-7412.

［4］Wang RB, Liu X. Development of DNA topoisomerase Ⅱ inhibitors［J］. Strait Pharmaceutical Journal, 2009, 22(4):1-5.（in Chinese）

王汝冰, 刘潇. DNA拓扑异构酶Ⅱ抑制剂研究进展［J］. 海峡药学, 2009, 22(4):1-5.

［5］Schutte NS,Malouff JM.A meta-analytic review of the effects of mindfulness meditation on telomerase activity ［J］. Psychoneuroendocrinology,2014, 42(1): 45-48.

［6］Gomez DE,Armando RG,Farina HG,et al.Telomere structure and telomerase in health and disease(review)［J］. Int J Oncol,2012, 41(5): 1561-1569.

［7］Ji FJ, Tian XF, Liu XW, et al. Dihydromyricetin induces cell apoptosis via a p53-related pathway in AGS human gastric cancer cells［J］. Genet Mol Res, 2015, 14(4):15564-15571.

［8］Liu B, Tan X, Liang J, et al. A reduction in reactive oxygen species contributes to dihydromyricetin-induced apoptosis in human hepatocellular carcinoma cells［J］. Sci Rep, 2014, 4:7041.

［9］Wang Z, Sun X, Feng Y, et al. Dihydromyricetin reverses MRP2-mediated MDR and enhances anticancer activity induced by oxaliplatin in colorectal cancer cells［J］. Anticancer Drugs, 2017, 28(3):281-288.

［10］Zhou DZ, Sun HY, Yue JQ, et al. Dihydromyricetin induces apoptosis and cytoprotective autophagy through ROS-NF-κB signalling in human melanoma cells［J］. Free Radic Res, 2017, 51(5):517-528.

［11］Xu Y, Wang S, Chan HF, et al. Dihydromyricetin induces apoptosis and reverses drug resistance in ovarian cancer cells by p53-mediated downregulation of survivin［J］. Sci Rep, 2017, 7:46060.

［12］Guo QQ, Yuan J, Zeng JH, et al. Synthesis of dihydromyricetin-manganese (ii) complex and interaction with DNA ［J］. J Mol Struct, 2012, 1027(43): 64-69.

［13］Hou XL, Wang WQ, Shi ChY, et al. Research progress in pharmacological effects of dihydromyricelin［J］. Chinese Traditional and Herbal Drugs, 2015, 46(4):603-609.（in Chinese）

侯小龙, 王文清, 施春阳,等. 二氢杨梅素药理作用研究进展［J］. 中草药, 2015, 46(4):603-609.

［14］Shan ChM，Liu TSh，Ma Y, et al.Study on the expressions of genes bcl-2,bax and apoptosis in human cholangicellular carcinomas［J］.Acta Anatomica Sinica,2002, 33(4):405-409.（in Chinese）

单长民, 刘同慎, 马云，等肝胆管癌中bcl-2、bax基因表达和细胞凋亡的研究［J］. 解剖学报, 2002, 33(4):405-409.

［15］Wu S, Liu B, Zhang Q, et al. Dihydromyricetin reduced bcl-2 expression via p53 in human hepatoma hepg2 cells［J］. PLoS One, 2013, 8(11): e76886.

［16］Lu CJ,He YF, Yuan WZ, et al.Dihydromyricetin-mediated inhibition of the Notch1 pathway induces apoptosis in QGY7701 and HepG2 hepatoma cells［J］.World J Gastroenterol,2017, 23(34): 6242-6251.

［17］Zhang Q,Liu DY. Ampelopsin induces apoptosis via altering expression of Bcl-2/Bax and activating Caspase-3 in human hepatoma cell line Bel-7402［J］. Chinese Pharmacological Bulletin, 2009, 25(11): 1502-1506.（in Chinese）

张琼, 刘德育. 蛇葡萄素改变Bcl-2/Bax 表达和激活Caspase-3 诱导人肝癌细胞Bel-7402 凋亡［J］. 中国药理学通报, 2009, 25(11): 1502-1506.

［18］V?lp K, Brezniceanu M L, B?sser S, et al. Increased expression of high mobility group box 1 (HMGB1) is associated with an elevated level of the antiapoptotic c-IAP2 protein in human colon carcinomas［J］. Gut, 2006, 55(2):234-242.

［19］Yong-Xian G, Xiao-Huan L, Fan Z, et al. Gemcitabine inhibits proliferation and induces apoptosis in human pancreatic cancer PANC-1 cells［J］. J Cancer Res Ther, 2016, 12(Suppl):1-4.