Expression of ephrin A3 during valproate induced neural stem cells differentiation

LI Wen HE Hui PENG Min QIN Jian-bing SHI Jin-hong JIN Guo-hua*

doi:10.16098/j.issn.0529-1356.2018.03.004

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Acta Anatomica Sinica ›› 2018, Vol. 49 ›› Issue (3) : 294-298. DOI: 10.16098/j.issn.0529-1356.2018.03.004

Neurobiology

Expression of ephrin A3 during valproate induced neural stem cells differentiation

LI Wen HE Hui PENG Min QIN Jian-bing SHI Jin-hong JIN Guo-hua*

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Abstract

Objective To investigate the expression of ephrin A3 (Efna3) in the process of neural stem cells differentiation induced by valproate (VPA) and the expression pattern of Efna3 in the nervous system. Methods The hippocampal neural stem cells of SD rats were isolated and cultured. At 24 hours and 48 hours after VPA inducement, the dynamic expression of Efna3 was detected by Real-time PCR, Western blotting and immunofluoresence assay. Real-time PCR was used to detect the expression of Efna3 in various tissues, as well as neural stem cells, astrocytes and neurons of adult SD rats. Results The expression of Efna3 increased significantly in the VPA group compared to the control group. Efna3 showed dominant expression in the cerebrum and hippocampus, and its expression was higher in neurons and lower in astrocytes. Conclusion The promotion of VPA to the differentiation of neural stem cells may be related to the upregulation of Efna3.

Key words

Ephrin A3 / Valproate / Neural stem cell / Neuron / Real-time PCR / Rat

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LI Wen HE Hui PENG Min QIN Jian-bing SHI Jin-hong JIN Guo-hua. Expression of ephrin A3 during valproate induced neural stem cells differentiation[J]. Acta Anatomica Sinica. 2018, 49(3): 294-298 https://doi.org/10.16098/j.issn.0529-1356.2018.03.004

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