Distribution and expression changes of the vesicular glutamate transporter 1 and vesicular glutamate transporter 2 immunopositive fibers and terminals in the postnatal development of the spinal cord of rat

LI Jing CHEN Tao LI Jin-lian*

doi:10.16098/j.issn.0529-1356.2018.03.003

PDF(765 KB)

Acta Anatomica Sinica ›› 2018, Vol. 49 ›› Issue (3) : 288-293. DOI: 10.16098/j.issn.0529-1356.2018.03.003

Distribution and expression changes of the vesicular glutamate transporter 1 and vesicular glutamate transporter 2 immunopositive fibers and terminals in the postnatal development of the spinal cord of rat

LI Jing¹ CHEN Tao² LI Jin-lian^2*

Author information +

History +

Abstract

Objective To explore different distribution and expression changes of vesicular glutamate transporter 1(VGLUT1) and VGLUT2 immunopositive (-ip) fibers and terminals in the spinal cord of postnatal 0 day (P0)-22 days (P22) rats. Methods Immunohistochemical staining was performed for the VGLUT1 and VGLUT2 in the cervical and lumber enlargements of P0-P22 rats. Results VGLUT1-and VGLUT2-ip fibers and terminals were observed in the cervical and lumber enlargements of the spinal cord in the postnatal P0-P22 rats but not soma. These fibers and termianls showed a typical complementary distribution pattern, especially in the posterior horn of the spinal cord. On P0, VGLUT1-ip fibers and terminals were mainly observed in the laminae Ⅲ-Ⅴ of the cervical and lumber enlargements, with a weak expression in the intermediate gray and anterior horn. From P3, the expression of VGLUT1 was increased in the laminae Ⅲ-Ⅴ and extended to the lateral, and the VGLUT1-ip profiles were detected in the basal part of posterior horn (lamina Ⅵ) and the lateral part of the anterior horn (lamina Ⅳ). An obvious trend of the belt distribution from the dorsomedial to ventrolateral side was observed. It was more obvious at P7, and expanded gradually to the medial and lateral side of spinal grey with development. On P22, VGLUT1-ip profiles were observed on almost whole spinal gray, except for a week expression in the lamina Ⅱ. On the contrary, VGLUT2-ip fibers and terminals were observed to distribute densely in laminae I-Ⅱ and the margins of the anterior horn from P0. In the other areas of the spinal cord, medium density of VGLUT2-ip fibers and terminals were distributed much more evenly. With the development, the distribution pattern of VGLUT2-ip fibers and terminals were not changed greatly, although its density was gradually increased. On the other hand, in the posterior cord of the spinal white matter, the developmental process of the VGLUT1 positive corticospinal posterior tract gradually went down from the cervical (P3) to the lumbar cord (P7). Conclusion The distribution of VGLUT1-and VGLUT2-ip fibers and terminals is largely different in the postnatal developing cervical and lumber spinal cord of rats, showing an obvious complementary pattern. This may be important for us to understand the difference of their functional involvement during the postnatal development of the spinal cord.

Key words

Postnatal development

/ Spinal cord / Vesicular glutamate transporter 1 / Vesicular glutamate transporter 2 / Immunohistochemistry / Rat

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LI Jing CHEN Tao LI Jin-lian.

[J]. Acta Anatomica Sinica. 2018, 49(3): 288-293 https://doi.org/10.16098/j.issn.0529-1356.2018.03.003

References

［1］Ni B, Rosteck PR, Jr., Nadi NS, et al. Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter［J］. Proc Natl Acad Sci USA, 1994, 91(12): 5607-5611.

［2］Aihara Y, Mashima H, Onda H, et al. Molecular cloning of a novel brain-type Na(+)-dependent inorganic phosphate cotransporter［J］. J Neurochem, 2000, 74(6): 2622-2625.

［3］Kaneko T, Fujiyama F. Complementary distribution of vesicular glutamate transporters in the central nervous system［J］. Neurosci Res, 2002, 42(4): 243-250.

［4］Alvarez FJ, Villalba RM, Zerda R, et al. Vesicular glutamate transporters in the spinal cord, with special reference to sensory primary afferent synapses［J］. J Comp Neurol, 2004, 472(3): 257-280.

［5］Brumovsky P, Watanabe M, H?kfelt T. Expression of the vesicular glutamate transporters-1 and-2 in adult mouse dorsal root ganglia and spinal cord and their regulation by nerve injury［J］. Neuroscience, 2007, 147(2): 469-490.

［6］Oliveira AL, Hydling F, Olsson E, et al. Cellular localization of three vesicular glutamate transporter mRNAs and proteins in rat spinal cord and dorsal root ganglia［J］. Synapse, 2003, 50(2): 117-129.

［7］Bannatyne BA, Edgley SA, Hammar I, et al. Differential projections of excitatory and inhibitory dorsal horn interneurons relaying information from group Ⅱ muscle afferents in the cat spinal cord［J］. J Neurosci, 2006, 26(11): 2871-2880.

［8］Landry M, Bouali-Benazzouz R, El Mestikawy S, et al. Expression of vesicular glutamate transporters in rat lumbar spinal cord, with a note on dorsal root ganglia［J］. J Comp Neurol, 2004, 468(3): 380-394.

［9］Malet M, Brumovsky PR. VGLUTs and glutamate synthesis-focus on DRG neurons and pain［J］. Biomolecules, 2015,5(4): 3416-3437.

［10］Qu JL，Li JL，Xiong KH, et al. Effects of VGLUT1 postive neurons in the lumbar segment of dorsal root ganglion after the complete ligation of unilateral sciatic nerve of the rat［J］．Chinese Journal of Neuroanatomy, 2006, 22(3):286-290.（in Chinese）

屈金良, 李金莲, 熊抗辉, 等. 单侧坐骨神经完全结扎对大鼠腰段背根神经节内VGluT1阳性神经元的影响［J}. 神经解剖学杂志, 2006, 22(3):286-290.

［11］Wang ZT, Yu G, Wang HS, et al. Changes in VGLUT2 expression and function in pain-related supraspinal regions correlate with the pathogenesis of neuropathic pain in a mouse spared nerve injury model［J］. Brain Res, 2015, 1624: 515-524.

［12］Gebre SA, Reeber SL, Sillitoe RV. Parasagittal compartmentation of cerebellar mossy fibers as revealed by the patterned expression of vesicular glutamate transporters VGLUT1 and VGLUT2［J］. Brain Struct Funct, 2012, 217(2):165-180.

［13］Wang HS, Yu G, Wang ZT, et al. Changes in VGLUT1 and VGLUT2 expression in rat dorsal root ganglia and spinal cord following spared nerve injury［J］. Neurochem Int, 2016,99: 9-15.

［14］Fremeau RT Jr, Kam K, Qureshi T, et al. Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites［J］. Science, 2004, 304(5678): 1815-1819.

［15］Malet M, Brumovsky PR. VGLUTs and glutamate synthesis-focus on DRG neurons and pain［J］. Biomolecules, 2015, 5(4): 3416-3437.

［16］Lagerstrom MC, Rogoz K, Abrahamsen B, et al. VGLUT2-dependent sensory neurons in the TRPV1 population regulate pain and itch［J］. Neuron, 2010,68(3): 529-542.

［17］McCarthy CJ, Tomasella E, Malet M, et al. Axotomy of tributaries of the pelvic and pudendal nerves induces changes in the neurochemistry of mouse dorsal root ganglion neurons and the spinal cord［J］. Brain Struct Funct, 2016, 221(4):1985-2004.

［18］Aresh B, Freitag FB, Perry S, et al. Spinal cord interneurons expressing the gastrin-releasing peptide receptor convey itch through VGLUT2-mediated signaling［J］. Pain, 2017,158(5):945-961.