Objective To explore the polymorphisms of rs3819024 and rs1974226 in interleukin-17A (IL-17A) gene in Guangxi populations, and to compare their genotypes and allele frequency with different races and region populations. Methods The polymorphisms of rs3819024 and rs1974226 of 443 Guangxi participants were detected by SNaPshot SNP and DNA sequencing method . Statistical method was used to compare the distribution difference between the genotype and allele frequency of rs3819024 and rs1974226. Four populations (HapMap-CHB, HapMap-JPT, HapMap-CEU, HapMap-YRI) were studied and published by the international HapMap project. Results The rs3819024 polymorphism contained GG, GA and AA genotypes respectively, while the rs1974226 polymorphism contained CC, CT and TT genotypes respectively in Guangxi population. The genotype and allele frequency of rs3819024 and rs1974226 were not significantly different between genders in Guangxi population (P>0.05). The genotype and allele frequency of the rs3819024 polymorphism in Guangxi population were not significantly different from HapMap-HCB (P>0.05), but were significantly different from HapMap-JPT, HapMap-CEU and HapMap-YRI population (P<0.01).The genotype and allele frequency of the rs1974226 polymorphism in Guangxi population were not significantly different from HapMap-HCB and HapMap-JPT population (P>0.05), but were significantly different from HapMap-CEU and HapMap-YRI population (P<0.05). Conclusion There are IL-17A rs3819024 and rs1974226 polymorphisms in Guangxi population, and there are varying degrees of difference in genotype and allele distribution of the two polymorphisms between Guangxi population and different other races and region populations.