Effect and mechanism of trefoil factor 3 on epithelial-mesenchymal transition of papillary thyroid carcinoma TPC-1 cells

LIN Xu WANG Zhi-yong WU Jing-fang* ZHANG Wen-jing ZHANG Jing XUE Gang*

doi:10.16098/j.issn.0529-1356.2018.02.011

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Acta Anatomica Sinica ›› 2018, Vol. 49 ›› Issue (2) : 204-211. DOI: 10.16098/j.issn.0529-1356.2018.02.011

Effect and mechanism of trefoil factor 3 on epithelial-mesenchymal transition of papillary thyroid carcinoma TPC-1 cells

LIN Xu¹ WANG Zhi-yong¹WU Jing-fang ^1* ZHANG Wen-jing¹ ZHANG Jing¹XUE Gang ^2*

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Abstract

Objective To explore the roles and mechanisms of silence trefoil factor 3(TFF3) on migration, invasion,clony formation and epithelial-mesenchymal transition of thyroid papillary carcinoma TPC-1 cells. Methods Expressions of Snail and TFF3 in tissue microarrays of thyroid papillary carcinoma were analysed by immunohistochemistry. Invasion assays, wound healing assays and colony formation tests were performed to detect the effects of TFF3 on migration, invasion and clony formation of TPC-1 cells. Epithelial-mesenchymal transition(EMT) markers and regulatory molecules were detected by Real-time PCR assay,Western blotting and immunocytochemistry in TPC-1 cells and mitogen-activated protein kinase (MAPK) signalling pathway related proteins were examined by Western blotting and immunocytochemistry. Results In 31 cases of papillary thyroid carcinoma, Snail and TFF3 positive signals were observed in the cytoplasm of papillary throid carcinoma(PTC),and were negative in the para-carcinoma.The expression of Snail in papillary thyroid carcinoma showed positive correlation with the expression of TFF3 protein(r=0.8450,P<0.05). Siliencing TFF3 expression markedly inhibited the ability of migration，invasion and clony formation in vitrovia suppressing of the MAPK signalling pathway and inhibited the process of EMT(P<0.05 or P<0.01). Conclusion Knockdown of TFF3 inhibits TPC-1 cells migration,invasion clony formation and EMT related proteins epression through suppressing MAPK/ERK signalling pathway.

Key words

Trefoil factor 3 / Epithelial-mesenchymal transition / Immunohistochemistry / Western blotting / Real-time PCR / Human

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LIN Xu WANG Zhi-yong WU Jing-fang ZHANG Wen-jing ZHANG Jing XUE Gang. Effect and mechanism of trefoil factor 3 on epithelial-mesenchymal transition of papillary thyroid carcinoma TPC-1 cells[J]. Acta Anatomica Sinica. 2018, 49(2): 204-211 https://doi.org/10.16098/j.issn.0529-1356.2018.02.011

References

［1］Meng L, Hu S, Huang L, et al.Papillary thyroid cancer coexisting with thyroid tuberculosis: a case report［J］. Oncol Lett, 2014, 7(5):1563-1565.

［2］Yoon SG, Yi JW, Seong CY, et al.Clinical characteristics of papillary thyroid carcinoma arising from the pyramidal lobe［J］. Ann Surg Treat Res, 2017, 92(3):123-128.

［3］Illam SP, Narayanankutty A, Mathew SE, et al. Epithelial mesenchymal transition in cancer progression: preventive Phytochemicals［J］. Recent Pat Anticancer Drug Discov, 2017, 12(3):234-246.

［4］May FE, Westley BR.TFF3 is a valuable predictive biomarker of endocrine response in metastatic breast cancer［J］. Endocr Relat Cancer, 2015, 22(3):465-479.

［5］Li Y, Sun Z, Liu K, et al.Prognostic significance of the co-expression of nucleophosmin and trefoil factor 3 in postoperative gastric cancer patients［J］. Mol Clin Oncol, 2014, 2(6):1055-1061.

［6］Xiao L, Liu YP, Xiao CX, et al.Serum TFF3 may be a pharamcodynamic marker of responses to chemotherapy in gastrointestinal cancers［J］. BMC Clin Pathol, 2014, 14(1):26.

［7］Zhang XJ,Wu JF,Huang J,et al. Expression and clinical significance of trefoil factor 3 in patiens with papillary thyroid carcinoma［J］.Acta Anatomica Sinica, 2013(4):503-508. (in Chinese)

张小俊, 吴靖芳, 黄静, 等.三叶因子3在甲状腺乳头状癌中的表达及临床意义［J］. 解剖学报, 2013(4):503-508.

［8］Wu JF. Study on the effect and mechanism of RNA inference targeting TFF3 gene on the proliferation and invasion ability of papillary carcinoma cells［D］. Hebei Medical University, 2016. (in Chinese)

吴靖芳. RNA干扰TFF3基因对人甲状腺乳头状癌细胞增殖与侵袭的影响及机制研究［D］. 河北医科大学, 2016.

［9］You ML, Chen YJ, Chong QY, et al.Trefoil factor 3 mediation of oncogenicity and chemoresistance in hepatocellular carcinoma is AKT-BCL-2 dependent［J］. Oncotarget, 2017, 8(24):39323-39344.

［10］Khoury T, Chadha K, Javle M, et al.Expression of intestinal trefoil factor (TFF-3) in hepatocellular carcinoma［J］. Int J Gastrointest Cancer, 2005, 35(3):171-177.

［11］Pandey V, Wu ZS, Zhang M, et al.Trefoil factor 3 promotes metastatic seeding and predicts poor survival outcome of patients with mammary carcinoma［J］. Breast Cancer Res, 2014, 16(5):429.

［12］Yuan Z, Chen D, Chen X, et al.Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells［J］. Cancer Cell Int, 2017, 17(1):7.

［13］Hoffmann W.Trefoil factors TFF (trefoil factor family) peptide-triggered signals promoting mucosal restitution［J］. Cell Mol Life Sci, 2005, 62(24):2932-2938.

［14］Kannan N, Kang J, Kong X, et al.Trefoil factor 3 is oncogenic and mediates antiestrogen resistance in human mammary carcinoma［J］. Neoplasia, 2010, 12(12):1041-1053.

［15］Lamouille S, Xu J, Derynck R.Molecular mechanisms of epithelial-mesenchymal transition［J］. Nat Rev Mol Cell Biol, 2014, 15(3):178-196.

［16］Romano S, Staibano S, Greco A, et al.FK506 binding protein 51 positively regulates melanoma stemness and metastatic potential［J］. Cell Death Dis, 2013, 4 (4):e578.

［17］Saxena M, Stephens MA, Pathak H, et al.Transcription factors that mediate epithelial-mesenchymal transition lead to multidrug resistance by upregulating ABC transporters［J］. Cell Death Dis, 2011, 2 (7):e179.

［18］Zhao XL, Sun T, Che N, et al.Promotion of hepatocellular carcinoma metastasis through matrix metalloproteinase activation by epithelial-mesenchymal transition regulator Twist1［J］. J Cell Mol Med, 2011, 15(3):691-700.

［19］Li S, Lu J, Chen Y, et al.MCP-1-induced ERK/GSK-3beta/Snail signaling facilitates the epithelial-mesenchymal transition and promotes the migration of MCF-7 human breast carcinoma cells［J］. Cell Mol Immunol, 2017, 14(7):621-630.

［20］Zhou Q, Chen J, Feng J, et al. SOSTDC1 inhibits follicular thyroid cancer cell proliferation, migration, and EMT via suppressing PI3K/Akt and MAPK/Erk signaling pathways［J］. Mol Cell Biochem, 2017, 435 (1-2):87-95.

［21] Baus-Loncar M, Giraud A. SMultiple regulatory pathways for trefoil factor (TFF) genes［J］. Cell Mol Life Sci, 2005, 62(24):2921-2931.

［22］Lin J, Sun Z, Zhang W, et al.Protective effects of intestinal trefoil factor (ITF) on gastric mucosal epithelium through activation of extracellular signal-regulated kinase 1/2 (ERK1/2)［J］. Mol Cell Biochem, 2015, 404(1-2):263-270.

［23］Xing M.Molecular pathogenesis and mechanisms of thyroid cancer［J］. Nat Rev Cancer, 2013, 13(3):184-199.