Expression of transforming growth factor-&beta; and Smad4 in ovarian granulosa cells in menopausal transitional rat

YUAN Li-juan REN Jun-xu* JI Hong-yu ZHANG Jing ZHANG Juan DING Yan

doi:10.16098/j.issn.0529-1356.2018.01.018

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Acta Anatomica Sinica ›› 2018, Vol. 49 ›› Issue (1) : 108-112. DOI: 10.16098/j.issn.0529-1356.2018.01.018

Histology,Embryology and Developmental Biology

Expression of transforming growth factor-β and Smad4 in ovarian granulosa cells in menopausal transitional rat

YUAN Li-juan¹REN Jun-xu ^2* JI Hong-yu³ZHANG Jing² ZHANG Juan¹DING Yan¹

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Abstract

Objective To investigate the expression of transforming growth factor beta(TGF-β) and Smad4 in ovarian granulosa cells of rats during menopausal transition and to analyze their relationship with the decline of ovarian function. Methods Female SD rats were divided into control group (group C, 6 months old, vaginal smear screening, n=9) and menopausal transition(MT) group (MT group, 12-14 months old, vaginal smear screening, n=8), rats were sacrificed quickly after ovariectomy. The ovarian granulosa cells were released by mechanical separation method, and cultured in CO₂ incubator. The expression of follicle-stimulating hormone receptor(FSHR) protein was observed by immunocytochemistry to identify ovarian granulosa cells. Immunohistochemistry was used to detect the expression of TGF-β and Smad4 protein in ovarian granulosa cells and images were collected and analyzed; The expression of TGF-β and Smad4 mRNA in granulosa cells was detected by Real-time PCR. Results Immunocytochemistry showed that the purity of granulosa cells was > 95%. The intensities of immunocytochemical positive staining for TGF-β and Smad4 in MT group was lower than those in normal group (P<0.05).Real-time PCR showed that both TGF-β and Smad4 mRNA were expressed. The amount of Real-time PCR for TGF-β and Smad4 mRNA was significantly lower than those of the control group (P<0.05). Conclusion TGF-β and Smad4 may be involved in the decline of ovarian function in rats with menopausal transition. The causes of the functional decline in postmenopausal rats may be partly related to the decrease of TGF-β and Smad4 expression in ovarian granulosa cells.

Key words

Menopausal transitional / Ovarian granulosa cells / Transforming growth factor-β / Smad4 / Immunohis tochemistry / Real-time / PCR / Rat

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YUAN Li-juan REN Jun-xu JI Hong-yu ZHANG Jing ZHANG Juan DING Yan. Expression of transforming growth factor-β and Smad4 in ovarian granulosa cells in menopausal transitional rat[J]. Acta Anatomica Sinica. 2018, 49(1): 108-112 https://doi.org/10.16098/j.issn.0529-1356.2018.01.018

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