Effects of dihydroartemisinin on proliferation and apoptosis of human pancreatic cancer cell line

ZHU Wen-he XU Na XU Jun-jie ZHANG Hong LI Yan Lü Shi-jie ZHANG Wei*

doi:10.16098/j.issn.0529-1356.2018.01.011

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Acta Anatomica Sinica ›› 2018, Vol. 49 ›› Issue (1) : 70-74. DOI: 10.16098/j.issn.0529-1356.2018.01.011

Cancer Biology

Effects of dihydroartemisinin on proliferation and apoptosis of human pancreatic cancer cell line

ZHU Wen-he XU Na XU Jun-jie ZHANG Hong LI Yan Lü Shi-jie ZHANG Wei*

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Abstract

Objective To investigate the effect of dihydroartemisinin on proliferation and apoptosis of human pancreatic cancer cell (PANC-1) and explore its mechanism.Methods After PANC-1cells treated with 30, 60, 120, 240 and 480 μmol/L dihydroartemisinin for 48 hours, MTT assay was used to detect the proliferation effect of dihydroartemisinin on PANC-1 cells. The cell cycle and apoptosis were detected by flow cytometry. The expressions of cyclin B1, Cdk1, p21 and apoptosis related protein Bcl-2, Bax, Caspase-3, Caspase-9 and cytochrome C(Cyto C) were detected by Western blotting. Results MTT result showed that the proliferation of PANC-1 cells was inhibited obviously after treatment with dihydroartemisinin by the concentration of 30-480 μmol/L (P<0.05). The result of flow cytometry showed that dihydroartemisinin could block PANC-1 cell cycle in G₂/M phase and induce cells apoptosis (P<0.05). The result of Western blotting showed that the expressions of Cdk1 and cyclin B1 were decreased and the expression of P21 increased after treatment with dihydroartemisinin. The expression of Bcl-2 was downregulated, expression of Bax was up-regulated, the ration of Bax/Bcl-2 was increased and the expression of cleaved Caspase-3, cleaved Caspase-9 and Cyto C were up-regulated. Conclusion Dihydroartemisinin can inhibit the proliferation of PANC-1 cells and induce apoptosis. The possible mechanism might be related to mitochondrial pathway.

Key words

Dihydroartemisinin / Pancreatic cancer / G₂/M block / Western blotting / Human

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ZHU Wen-he XU Na XU Jun-jie ZHANG Hong LI Yan Lü Shi-jie ZHANG Wei. Effects of dihydroartemisinin on proliferation and apoptosis of human pancreatic cancer cell line[J]. Acta Anatomica Sinica. 2018, 49(1): 70-74 https://doi.org/10.16098/j.issn.0529-1356.2018.01.011

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