Expression of DDX3 and casein kinase 1&epsilon; in the brain stem of amyotrophic lateral sclerosis transgenic mice

ZHANG Ya-wen WANG Qing YUAN Meng LIU Huan-cai WANG Qiao-zhen DING Hao-yu ZHOU Feng-hua CHEN Yan-chun*

doi:10.16098/j.issn.0529-1356.2018.01.001

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Acta Anatomica Sinica ›› 2018, Vol. 49 ›› Issue (1) : 1-6. DOI: 10.16098/j.issn.0529-1356.2018.01.001

Neurobiology

Expression of DDX3 and casein kinase 1ε in the brain stem of amyotrophic lateral sclerosis transgenic mice

ZHANG Ya-wen ^1,2 WANG Qing³YUAN Meng² LIU Huan-cai⁴WANG Qiao-zhen³ DING Hao-yu² ZHOU Feng-hua² CHEN Yan-chun ^2*

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Abstract

Objective To detect the expression of DDX3 and casein kinase 1ε (CK1ε）in the brain stem of amyotrophic lateral sclerosis (ALS) transgenic mice and study the role of DDX3 and CK1ε in the degeneration of brain stem motor neurons of amyotrophic lateral sclerosis (ALS). Methods Thirty-three ALS transgenic mice were used in this study. The brain stem was dissected and collected at the early (95 days), middle (108 days) or late (122 days) stages. The expression of DDX3 and CK1ε in the brain stem of ALS transgenic mice, the distribution and co-localization of positive cells in the hypoglossal nucleus and facial nucleus of the brain stem were detected by RT-PCR, Western blotting and immunofluorescence technology. In each group, the same number of wild type littermates were selected as controls. Results The result of RT-PCR and Western blotting showed that compared with the wild type mice, the expression of DDX3 and CK1ε mRNA in the brain stem of ALS mice remained unchanged at day 95, day 108 and day 122. DDX3 and CK1ε protein levels were up-regulated at day 95 and day 108 but down-regulated at day 122 in the ALS mice brain stem group (P<0.01, P<0.001). The result of immunofluorescence showed that DDX3 and CK1ε positive cells were detected in the sublingual nerve and facial nerve of ALS mice and wild type mice brain stem. DDX3 and CK1ε were expressed in neurons, but not in astrocytes. The immunoreactivity of both DDX3 and CK1ε of ALS mice and wild type mice was different. Conclusion The abnormal expression of DDX3 and CK1ε in the brain stem is closely related to the pathogenesis of ALS.

Key words

Amyotrophic lateral sclerosis / Brain stem / DDX3 / Casein kinase 1ε / Western blotting / ＲT-PCＲ / Mouse

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ZHANG Ya-wen WANG Qing YUAN Meng LIU Huan-cai WANG Qiao-zhen DING Hao-yu ZHOU Feng-hua CHEN Yan-chun. Expression of DDX3 and casein kinase 1ε in the brain stem of amyotrophic lateral sclerosis transgenic mice[J]. Acta Anatomica Sinica. 2018, 49(1): 1-6 https://doi.org/10.16098/j.issn.0529-1356.2018.01.001

References

［1］Paez-Colasante X, FigueroaRomero C, Sakowski SA, et al. Amyotrophic lateral sclerosis: mechanisms and therapeutics in the epigenomic era ［J］. Nat Rev Neurol,2015,11(5):266-279.

［2］Renton AE,Chiò A,Traynor BJ. State of play in amyotrophic lateral sclerosis genetics ［J］. Nat Neurosci, 2014,17(1):17-23.

［3] K ühnlein P,Gdynia HJ, Sperfeld AD, et al. Diagnosis and treatment of bulbar symptoms in amyotrophic lateral sclerosis ［J］. Nat Clin Pract Neurol, 2008,4(7):366-374.

［4］Oliver D. The quality of care and symptom control-the effects on the terminal phase of ALS/MND ［J］. J Neurol Sci, 1996,139(Supp1):134-136.

［5］Oh SI, Park A, Kim HJ, et al. Spectrum of cognitive impairment in Korean ALS patients without known genetic mutations ［J］. PLoS One, 2014, 9(2):e87163.

［6］Ferrucci M, Spalloni A, Bartalucci A, et al. A systematic study of brainstem motor nuclei in a mouse model of ALS, the effects of lithium ［J］.Neurobiol Dis, 2010, 37(2):370-383.

［7］González-Fernández C, Mancuso R, Del Valle J, et al.Wnt signaling alteration in the spinal cord of amyotrophic lateral sclerosis transgenic mice: special focus on frizzled-5 cellular expression pattern ［J］. PLoS One, 2016, 11(5): e0155867.

［8］Chen Y, Guan Y, Liu H,et al. Activation of the Wnt/β-catenin signaling pathway is associated with glial proliferation in the adult spinal cord of ALS transgenic mice ［J］. Biochem Biophys Res Commun, 2012, 420 (2): 397-403.

［9］Cruciat CM, Dolde C, de Groot RE, et al. RNA helicase DDX3 is a regulatory subunit of casein kinase 1 in Wnt-β-catenin signaling［J］. Science, 2013, 339(6126):1436-1441.

［10］Pu LD, Zhang YW, Wang Q, et al. Expression of DDX3 and casein kinase1ε in the hippocampus of amyotrophic lateral sclerosis transgenic mice［J］ Acta Anatomica Sinica, 2017, 48(4):375-380. (in Chinese)

蒲蕾东, 张雅雯, 王箐, 等. DDX3和酪蛋白激酶1ε在肌萎缩侧索硬化症转基因鼠海马中的表达［J］. 解剖学报. 2017，48(4):375-380.

［11］Yedavalli VS, Zhang N, Cai H, et al. Ring expanded nucleoside analogues inhibit RNA helicaseand intracellular human immunodeficiency virustype 1 replication ［J］. J Med Chem, 2008, 51(16):5043-5051.

［12］Sharma D, Jankowsky E. The Ded1/DDX3 subfamily of DEAD-box RNA helicases［J］. Crit Rev Biochem Mol Biol, 2014, 49(4):343-360.

［13］Chen HH, Yu HI, Tarn WY. DDX3 modulates neurite development via translationally activating an RNA regulon involved in rac1 activation［J］. J Neurosci, 2016, 36(38): 9792-9804.

［14］Toledo EM, Colombres M, Inestrosa NC. Wnt signaling in neuroprotection and stem cell differentiation ［J］. Prog Neurobiol, 2008, 86(3):281-296.

［15］Clevers H, Nusse R. Wnt/β-catenin signaling and disease ［J］. Cell, 2012, 149(6): 1192-1205.

［16］Flajolet M, He G, Heiman M, et al. Regulation of Alzheimer’s disease amyloid-beta formation by casein kinase I ［J］. Proc Natl Acad Sci USA, 2007, 104(10):4159-4164.

［17］Bischof J, Müller A, Fnder M, et al. Neurite outgrowth of mature retinal ganglion cells and PC12 cells requires activity of CK1δ and CK1ε［J］. PLoS One, 2011, 6(6):e20857

［18］Zhou F, Guan Y, Chen Y, et al. miRNA-9 expression is upregulated in the spinal cord of G93A-SOD1 transgenic mice ［J］. Int J Clin Exp Pathol, 2013, 6(9):1826-1838.