Objective To provide a simple, convenient, and safe anesthesia method for the M1 segment of middle cerebral artery occlusion model of rhesus monkeys. Methods Twenty five male rhesusmonkeys, with an average age of 7-9 years and an average body weight of 7-11 kg, were selected for the study. Sumianxin injection combined with ketamine 0.1 mg/kg was given by intramuscular injection before endotracheal intubation(ID：4.5-5.5^# ). Animals were then transported to a interventional operation room, where the intravenous access was established with the insertion of a urinary catheter. Mechanical ventilation was used during surgery, propofol was continuous injected in a speed of 2-4 mg/(kg·h), sumianxin-ketamine mixture could be given if necessary to maintain an adequate anesthesia depth. Injection dosage was adjusted according to some vital signs of rhesus like the body movements, physiological parameters, and the demand of surgery. Brain MRI examination was performed before and after thrombolysis. Anesthesia was stopped to let the animals have a spontaneous breath every time before MRI text. Heart rates, temperature, non-invasive blood pressure, SpO₂ were monitored the whole surgery. Blood samples were taken separately from the radial artery for blood gas analysis after femoral artery puncture and at the time point of thrombolysis. Results All the 25 animals awake soon after surgery, no animal have restlessness, respiratory depression, arrhythmia and other serious complications. Eighteen of themsurvived longer than 24 hours, only 7 died from serious cerebral hemorrhage or large cerebral infarction. Conclusion Endotracheal intubation general anesthesia is safe and practicable for rhesus monkeys undergoing interventional operation and MRI examination procedures.