Sheep mammary duct and alveolus casting

LIU Ming-xing Lü Hong-fa ZHANG Jin-peng DING Meng-yuan SHENG Shuang YANG Bing*

doi:10.16098/j.issn.0529-1356.2017.06.015

PDF(563 KB)

Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (6) : 721-725. DOI: 10.16098/j.issn.0529-1356.2017.06.015

Anatomy

Sheep mammary duct and alveolus casting

LIU Ming-xing Lü Hong-fa ZHANG Jin-peng DING Meng-yuan SHENG Shuang YANG Bing*

Author information +

History +

Abstract

Objective To establish a sheep mastitis model in order to investigate the three dimensional morphological structure of the sheep mammary duct and alveolus structure. Methods Three postpartum sheep were used to establish a model of the sheep mastitis.Staphyloccus aureus (10¹²CFU/L) was inoculated into the left mammary gland and the equal amount of physiological saline was injected into the right mammary gland as the control. The milk of left and right mammary gland was detected by Beijing mastitis test (BMT) in different times (12 hours, 24 hours, 36 hours, 48 hours). The both mammary ducts and alveoli were molded by a casting mould technique and observed by scanning electron microscopy (SEM). Reselts The result showed the lactiferous duct was the first grade，including 14 branches. There was no connection between duct branches. The intersection angle between mammary duct ramification and the spindle extension was less than 90 degrees. The SEM found that the surface of right mammary ducts was smooth. There were many mammary alveoli at the terminal duct. The mean diameter of left alveolus was (79.03±14.91)μm. There were many ostioles on the surface of the left mammary ducts. The surface of the left mammary ducts was rough and most alveoli were atrophic. The mean diameter of left alveolus was (42.89±17.17)μm. Conclusion Staphyloccus aureus may destroy the construction of the mammary gland.

Key words

Mastitis / Casting / Alveolus / Scanning electron microscopy / Goat

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LIU Ming-xing Lü Hong-fa ZHANG Jin-peng DING Meng-yuan SHENG Shuang YANG Bing. Sheep mammary duct and alveolus casting[J]. Acta Anatomica Sinica. 2017, 48(6): 721-725 https://doi.org/10.16098/j.issn.0529-1356.2017.06.015

References

［1］Zhang RJ. Sheep mastitis treatment and preventive measures ［J］. Journal of Animal Science and Veterinary Medicine, 2015,34(2):132-133.(in Chinese)

张瑞娟. 羊乳腺炎的临床症状及治疗措施［J］. 畜牧兽医杂志，2015，34（2）:132-133.

［2］Wang D, Wang Z, Yan Z, et al. Bovine mastitis Staphylococcus aureus: antibiotic susceptibility profile, resistance genes and molecular typing of methicillin-resistant and methicillin-sensitive strains in China［J］. Infect Genet Evol, 2015, 31（10）:9-16.

［3］Pu WX, Su Y, Li JX, et al. High incidence of oxacillin-susceptible mecA-positive staphylococcus aureus (OS-MRSA) associated with bovine mastitis in China［J］. PLoS One, 2014, 9(2):e88134-e88134.

［4］Jensen K, Gunther J, Talbot R, et al. Escherichia coli-and staphylococcus aureus-induced mastitis differentially modulate transcriptional responses in neighbouring uninfected bovine mammary gland quarters［J］. BMC Genomics, 2013, 14(1):397-402.

［5］Akers RM, Nickerson SC. Mastitis and its impact on structure and function in the ruminant mammary gland［J］. J Mammary Gland Biol Neoplasia, 2011, 16(4):275-289.

［6］Hossler FE, Lametschwandtner A, Kao R,et al. Microvascular architecture of mouse urinary bladder described with vascular corrosion casting, light microscopy, SEM, and TEM［J］. Microsc Microanal, 2013,19(6):1428-1435.

［7］Sun Y, Yuan LG, Zhao HT. Distribution and morphological characteristic of testis lobule micranginm in adult Tibetan plateau sheep［J］. Acta Anatomica Sinica, 2011,42(1):114-118. (in Chinese)

孙英，袁莉刚，赵海涛.成年高原藏羊睾丸小叶内微血管分布和形态特征［J］. 解剖学报，2011，42（1）：114-118.

［8］Kajbafzadeh AM, Sabetkish N, Sabetkish S, et al. Lung tissue engineering and preservation of alveolar microstructure using a novel casting method［J］. Biotech Histochem, 2015, 90(2):111-123.

［9］Khimmaktong W, Petpiboolthai H, Panyarachun B, et al. Study of curcumin on microvasculature characteristic in diabetic rat's liver as revealed by vascular corrosion cast/scanning electron microscope (SEM) technique［J］. J Med Assoc Thai,2012,95（Suppl 5）:133-141.

［10］Du XH, Liu X, Liu Y, et al. Observation on the architecture of subpleural pulmonary microvasculature in Bactrian camel（camelus bactrianus）under SEM［J］. Acta Anatomica Sinica,2016,37(1):87-90. (in Chinese)

杜晓华,刘霞,刘英,等. 双峰驼胸膜下肺微血管构筑的扫描电镜观察［J］. 解剖学报,2006,37(1):87-90.

［11］Walocha JA, Litwin JA, Bereza T, et al. Vascular architecture of human uterine cervix visualized by corrosion casting and scanning electron microscopy［J］. Hum Reprod, 2012, 27(3):727-732.

［12］Li J, Jiang J, Zhan XE. A preliminary study of the resin cast on the normal and cancerous female mammary duct and acinus system［J］. Chinese Journal of Experimental Surgery, 2011,3(18):236-238. (in Chinese)

李峻，姜军，詹新恩. 女性正常及癌变乳腺导管系统铸型的初步研究［J］. 中华实验外科杂志,2011，3（18）：236-238.

［13］Liu N, Wang KR, Sun G, et al. The product of cast specimens of the domestic pig normal breast duct system［J］. Journal of Inner Mongolia Agricultural University, 2009, 30(4):282-283. (in Chinese)

刘宁，王可人，孙光，等. 家猪正常乳腺导管系统铸型标本的制作［J］. 内蒙古农业大学学报，2009，30(4):282-283.

［14］Wang H, Yu G, Yu H, et al. Characterization of TLR2, NOD2, and related cytokines in mammary glands infected by Staphylococcus aureus in a rat model［J］. Acta Vet Scand,2015, 57:25.

［15］Ingman WV, Glynn DJ, Hutchinson MR. Mouse models of mastitis-how physiological are they［J］？ Int Breastfeed J,2015, 10:12.

［16］Perruchot MH, ArevaloTurrubiarte M, Dufreneix F. Mammary epithelial cell hierarchy in the dairy cow throughout lactation［J］. Stem Cells Dev，2016, 25(19):1407-1418.

［17］Liu N, Wang KR, Yao ShR, et al. Preliminary study of female mammary duct casting anatomy［J］. Chinese Journal of Breast Disease, 2011, 5(5):586-592. (in Chinese)

刘宁，王可人，姚树仁，等. 女性乳腺导管的铸型解剖学初步研究［J］. 中华乳腺病杂志，2011， 5（5）：586-592.

［18］Zhu CX, Wang Q, Deng QD, et al. Evaluation of mammary ductoscopy classification applied in bloody nipple discharge［J］. Chinese Journal of Breast Disease, 2011,2(5):171-179. (in Chinese)

朱彩霞，王颀，邓群娣，等. 纤维乳管镜检查分级在乳头血性溢液疾病诊断中的应用价值［J］. 中华乳腺病杂志，2011，2（5）：171-179.

［19］Akers RM, Nickerson SC. Mastitis and its impact on structure and function in the ruminant mammary gland［J］. J Mammary Gland Biol Neoplasia,2011, 16(4):275-289.

［20］Zhu YH. Pathological features of Staphylococcus aureus-induced cinical mastitis model in Chinese Holstein and preliminary immunohistochemistry research［D］. Yangzhou University, 2015:5. (in Chinese)

朱怡慧. 奶牛金黄色葡萄球菌诱导型临床乳腺炎的病理特征及初步免疫组化研究［D］. 扬州大学， 2015：5.

［21］Lawrence TLJ, Fowler VR. Mammary Gland Growth and Product Yield［M］. New York:CABI Publishing, 2002: 103-119.