Effects of miR-182 on the proliferation and invasion of human melanoma cell line A375 by targeting of cyclic adenosine monophophate responsive element binding protein 1

WANG Li-na* WANG Kai-bo ZHUANG Yong-can WANG Hong-lan

doi:10.16098/j.issn.0529-1356.2017.06.013

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (6) : 710-714. DOI: 10.16098/j.issn.0529-1356.2017.06.013

Effects of miR-182 on the proliferation and invasion of human melanoma cell line A375 by targeting of cyclic adenosine monophophate responsive element binding protein 1

WANG Li-na ^1* WANG Kai-bo²ZHUANG Yong-can¹ WANG Hong-lan¹

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Abstract

Objective To investigate the effects of miR-182 on the proliferation and invasion of human melanoma cell line A375 and to elucidate its action mechanisms. Methods miR-182 mimics or inhibitor were transfected into A375 cells by lipofectamine package, MTT was applied to detect the viability of A375 cells, transwell chamber assay was used to measure the invasion of A375 cells, the expression levels of cyclic adenosine monophosphate responsive element binding protein 1(CREB1) mRNA and protein were detected by Real-time PCR and Western blotting respectively. Results Compared with the untreated group, miR-182 silence significantly increased the proliferation and invasion ability of A375 cells and obviously decreased the expression levels of CREB1 mRNA and protein, P<0.01, but with opposite role for miR-182 overexpression. Conclusion The reinforcing effect of miR-182 silence on the proliferation and invasion of A375 cells may be related to the down-regulation of CREB1.

Key words

miR-182 / Proliferation / Invasion / Cyclic adenosine monophosphate responsive element binding protein 1 / Transfect / A375 cell / Real-time PCR / Human

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WANG Li-na WANG Kai-bo ZHUANG Yong-can WANG Hong-lan. Effects of miR-182 on the proliferation and invasion of human melanoma cell line A375 by targeting of cyclic adenosine monophophate responsive element binding protein 1[J]. Acta Anatomica Sinica. 2017, 48(6): 710-714 https://doi.org/10.16098/j.issn.0529-1356.2017.06.013

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