Effect of cod skin oligopeptide on the proliferation and apoptosis in human gastric cancer cells line SGC-7901 and its mechanism

WU Li-ping XU Li-yun HU Xiao-fei ZHANG Guo-qiang*

doi:10.16098/j.issn.0529-1356.2017.06.011

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (6) : 698-703. DOI: 10.16098/j.issn.0529-1356.2017.06.011

Effect of cod skin oligopeptide on the proliferation and apoptosis in human gastric cancer cells line SGC-7901 and its mechanism

WU Li-ping¹ XU Li-yun²HU Xiao-fei¹ZHANG Guo-qiang ^1*

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Abstract

Objective To explore the effect of cod skin oligopeptide(CSO) on the proliferation in human gastric carcinoma SGC-7901 cells. Methods After treatment of SGC-7901 cells with different concentrations of CSO, the cells were observed under the microscope and the viability of cells was detected by CCK-8 assay. Cell apoptosis and cell cycle were determined by Western blotting and flow cytometry. Results CSO significantly suppressed the proliferation of SGC-7901 cells in a time-and dose-dependent manner（P<0.05）. The cultured SGC-7901 cells were treated by CSO for 24 hours and 48 hours and 72 hours with the IC50of 156.90 g/L and 102.10 g/L and 73.13 g/L.A large number of nuclear fragmentation and apoptotic bodies were observed under fluorescence microscope after treatment by DAPI. After treatment with CSO for 24 hours, SGC-7901 cells showed increased at G₁ stage and cell cycle arrested at S/G₂ stage, and the cell apoptosis rate increased with the CSO. With the increase of CSO concentration, the expression of Caspase-3 and Caspase-9 was up-regulated, and the expression of Bcl-2 was down-regulated. Conclusion CSO can promote the apoptosis and inhibit the proliferation of SGC-7901 cells. Anti-cancer mechanism may be related with Caspase-3 and Caspase-9 up-regulating,and Bcl-2 was down-regulating.

Key words

Cod skin oligopeptide / Gastric cancer / SGC-7901 / Caspase-3 / Caspase-9 / Bcl-2 / Western blotting / Flow cytometry / Human

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WU Li-ping XU Li-yun HU Xiao-fei ZHANG Guo-qiang. Effect of cod skin oligopeptide on the proliferation and apoptosis in human gastric cancer cells line SGC-7901 and its mechanism[J]. Acta Anatomica Sinica. 2017, 48(6): 698-703 https://doi.org/10.16098/j.issn.0529-1356.2017.06.011

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