Distal-less homeobox 2/ forkhead box O3a distribution and expression in the development of rat cerebral cortex

MU Ya-ting LI Yan GUO Qiong* WANG Lei*

doi:10.16098/j.issn.0529-1356.2017.06.004

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (6) : 658-662. DOI: 10.16098/j.issn.0529-1356.2017.06.004

Neurobiology

Distal-less homeobox 2/ forkhead box O3a distribution and expression in the development of rat cerebral cortex

MU Ya-ting¹LI Yan²GUO Qiong ^2* WANG Lei ^3*

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Abstract

Objective To investigate the expression of distal-less homeobox 2(Dlx2) and forkhead box O3a(FoxO3a) in developing cortex of SD rats. Methods Real-time PCR and immunohistochemical staining were performed to study the changes in Dlx2/FoxO3a expression. Results Dlx2/FoxO3a mRNA were expressed in embryonic day 11(E11)-postnatal day 1(P1), while the expression peak of FoxO3a mRNA was significantly earlier than Dlx2 mRNA. The expression of Dlx2 mRNA was significantly increased at E15-E17, and this high level expression was maintained until P1. The expression of Dlx2/FoxO3a mRNA showed a consistent trend in E15-P1. At E19,Dlx2 gene showed high expression of mRNA but no protein expression. Conclusion Dlx2 and Fox03a genes are expressed in the cerebral cortex of the late embryos,and their expression trend is consistent.The distributions of the two genes are changed with the cortical stratification.

Key words

Distal-less homeobox 2

/ Forkhead box O3a / Cerebral cortex / Embryo development / Real-time PCR / Immunohistochemistry / Rat

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MU Ya-ting LI Yan GUO Qiong WANG Lei. Distal-less homeobox 2/ forkhead box O3a distribution and expression in the development of rat cerebral cortex[J]. Acta Anatomica Sinica. 2017, 48(6): 658-662 https://doi.org/10.16098/j.issn.0529-1356.2017.06.004

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