Effects of intracerebral transplantation of RMNE6 on the ischemic injury of the brain

WU Yun* ZHAI Xiao-yan YANG Li-wang XU Qun-yuan

doi:10.16098/j.issn.0529-1356.2017.06.001

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (6) : 635-641. DOI: 10.16098/j.issn.0529-1356.2017.06.001

Neurobiology

Effects of intracerebral transplantation of RMNE6 on the ischemic injury of the brain

WU Yun ^1,2* ZHAI Xiao-yan¹ YANG Li-wang¹ XU Qun-yuan²

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Abstract

Objective To select the suitable stem cells for cell replacement in stroke therapy. Methods The RMNE6 cells were transplanted into the normal brain and injured brain of the stroke model of rats (n=8). The fates of the RMNE6 cells in the normal brain and the injured brain, the effects on the ischemic volume and the improvements on the behavior of the rats were observed by immunofluorescence staining, behaviour testing and magnetic resonance imaging, compared with the placebo group (n=8) and the fibroblast group (n=8). Results After transplantation, the RMNE6 cells were able to survive in the normal and ischemic brain area. There was tumourigenesis in the ischemic brain transplanted with the RMNE6 cells. The cell transplantation had no effects on the size of the ischemic volume. The behavior of the model animals had no significiant improvement. Conclusion As far as the safety is concerned, the RMNE6 cells are not suitable for cell replacement in the stroke therapy and still need to be modified.

Key words

RMNE6 cell / Cell transplantation / Brain ischemia / Immunofluorescence / Magnetic resonance imaging / Rat

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WU Yun ZHAI Xiao-yan YANG Li-wang XU Qun-yuan. Effects of intracerebral transplantation of RMNE6 on the ischemic injury of the brain[J]. Acta Anatomica Sinica. 2017, 48(6): 635-641 https://doi.org/10.16098/j.issn.0529-1356.2017.06.001

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