Comparison of different effects between myocardial micro-environment and 5-azacytidine induced adipose mesenchymal stem cells into myocardial cells

GUO Zhi-kun CHANG Dan-yang LIU Ling-ling Li Qiong*

doi:10.16098/j.issn.0529-1356.2017.05.007

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (5) : 538-544. DOI: 10.16098/j.issn.0529-1356.2017.05.007

Comparison of different effects between myocardial micro-environment and 5-azacytidine induced adipose mesenchymal stem cells into myocardial cells

GUO Zhi-kun¹ CHANG Dan-yang¹LIU Ling-ling²Li Qiong^1*

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Abstract

Objective To explore the different effects between myocardial micro-environmentin vivo and 5-azacytidine(5-aza) in vitroinduced adipose-derived mesenchymal stem cells (ADMSCs) into myocardial cells. Methods The ADMSCs were isolated from the subcutaneous fatty tissue of the groin area of mice and cultured. Their surface makers and osteogenic and adipogenic differentiation ability were identified. The third-generation ADMSCs were randomly divided into two groups: a 5-aza induced in vitrogroup (for 3 weeks), and a myocardial transplantation in vivo group (for 1 week). Immunofluorescent staining was used to detect the expression of cardiac troponin T (cTnT). Results ADMSCs expressed cTnT in both groups. The expression rate in the 5-aza induced group after 3 weeks was (33.33 ± 3.79)%; the expression rate of the transplantation group after 1 week was (42.93 ± 4.04)%. Compared with the 5-aza induced group, the transplantation group had a higher efficiency of differentiation (P<0.05). Conclusion ADMSCs can differentiate into myocardial cells in both in vitro 5-aza induced group and in vivo myocardial transplantation induced group, but the differentiation efficiency is significantly higher by myocardial microenvironment in vivo than that chemically induced in vitro.

Key words

Cardiac troponin T / 5-azacytidine / Adipose mesenchymal stem cell / Myocardial micro-environment / Immunofluorescence / Mouse

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GUO Zhi-kun CHANG Dan-yang LIU Ling-ling Li Qiong. Comparison of different effects between myocardial micro-environment and 5-azacytidine induced adipose mesenchymal stem cells into myocardial cells[J]. Acta Anatomica Sinica. 2017, 48(5): 538-544 https://doi.org/10.16098/j.issn.0529-1356.2017.05.007

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