Hantavirus infection of astrocytes from cerebral cortex of newborn rats

HE Shuai HE Yang YANG Shou-jing*

doi:10.16098/j.issn.0529-1356.2017.05.002

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (5) : 504-510. DOI: 10.16098/j.issn.0529-1356.2017.05.002

Neurobiology

Hantavirus infection of astrocytes from cerebral cortex of newborn rats

HE Shuai¹HE Yang²YANG Shou-jing^3*

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Abstract

Objective The primary focus of this research was to use astrocytes from newborn Sprague-Dawley rats less than 24 h infected with Hantaan 76-118 (HTNV) or Seoul virus (SEOV) strain L99 as an infection model in vitro to examine astrocyte susceptibility to hantavirus infection. Methods Astrocytes susceptibility to hantavirus infection was examined by immunofluorescence microscopy, Western blotting and genereverse transcription PCR (RT-PCR). The expression of GFAP gene was detected by RT-PCR assay. Results HTNV or SEOV infection rapidly induced viral nucleocapsid protein (NP) and glial fibrillary acidic portein (GFAP) expressions in astrocytes. GFAP and NP in infected astrocytes were co-localized. Upregulation of S segment RNA and GFAP gene was confirmed.Conclusion Our findings indicate that HTNV or SEOV availably infects and activates astrocytes, and GFAP may be actively involved in regulating structural and functional integrity of the BBB,viral NP synthesis and replication.

Key words

汉坦病毒 / 大脑皮层 / 星形胶质细胞 / 核衣壳蛋白 / 胶质纤维酸性蛋白 / 免疫荧光 / 大鼠

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HE Shuai HE Yang YANG Shou-jing. Hantavirus infection of astrocytes from cerebral cortex of newborn rats[J]. Acta Anatomica Sinica. 2017, 48(5): 504-510 https://doi.org/10.16098/j.issn.0529-1356.2017.05.002

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