Comparing clinical outcome of different methods for cryopreservation of human cleavage stage embryos

KUAI Yan-rong WAN Sheng ZHANG Kai ZENG Cheng XUE Qing SHANG Jing HE Zhan-ju YANG Hui-xia XU Yang*

doi:10.16098/j.issn.0529-1356.2017.04.020

PDF(257 KB)

Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (4) : 482-487. DOI: 10.16098/j.issn.0529-1356.2017.04.020

Technology and Methodology

Comparing clinical outcome of different methods for cryopreservation of human cleavage stage embryos

KUAI Yan-rong¹ WAN Sheng¹ ZHANG Kai¹ ZENG Cheng¹ XUE Qing¹ SHANG Jing¹HE Zhan-ju² YANG Hui-xia¹ XU Yang^1*

Author information +

History +

Abstract

Objective To compare clinical outcome of different methods for cryopreservation of human cleavage stage embryos. Methods The data of 1331 cleavage stage frozen-thawed embryo transfer cycles including 431 slow-freezing cycles and 900 vitrification cycles were retrospectively analyzed in Pecking First Hospital from January 2011 to December 2015. The survival rate, intact embryo rate, clinical pregnancy rate, implantation rate, cycle cancel rate and miscarry rate of differentmethod for cryopreservation of human cleavage stage embryos were compared. Results The survival rate (92.53%), intact embryo rate (75.43%), clinical pregnancy rate (45.72%), and implantation rate (27.41%) of vitrification were all higher than those of slow-freezing (76.93%, 48.46%, 39.52%, and 19.88%, respectively; P<0.05), but there was no difference between the miscarriage rate (12.20%; P>0.05) and the cycle cancel (12.56%; P>0.05). The clinical pregnancy rate of transfer 0, 1, 2, 3 intact embryos after slow-freezing were 36.2%, 37.7%, 42.0%, and 44.3% (P>0.05), respectively, and those after vitrification were 20.5%, 42.3%, 48.8%, and 54.1% (P<0.05) respectively. Conclusion Vitrification is a more effective means of cryopreserving human cleavage stage embryo than conventional slow freezing. There is no effect of cell loss on the freezing embryo development potency after slow-freezing, but cell loss affects the freezing embryo development potency after vitrification.

Key words

Clinical pregnancy / Frozen thawed embryo transfer / Slow freezing / Vitrification / Human

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

KUAI Yan-rong WAN Sheng ZHANG Kai ZENG Cheng XUE Qing SHANG Jing HE Zhan-ju YANG Hui-xia XU Yang. Comparing clinical outcome of different methods for cryopreservation of human cleavage stage embryos[J]. Acta Anatomica Sinica. 2017, 48(4): 482-487 https://doi.org/10.16098/j.issn.0529-1356.2017.04.020

References

［1］Trounson A，Morthr L． Human pregnancy following cryopreservation，thawing and transfer of an eight-cell stage embryo［J］. Nature, 1983, 305(5903): 707-709．

［2］Li Z, Wang YA, Ledger W, et al. Clinical outcomes following cryopreservation of blastocysts by vitrification or slow freezing: a population-based cohort study［J］. Hum Reprod, 2014, 29(12): 2794-2801.

［3］Jacob L, Oshrit L, Masha B, et al.Cryopreservation of day 2-3 embryos by vitrification yields better outcome than slow freezing［J］. Gynecol Endocrinol, 2014; 30(3): 202-204.

［4］Mojtaba RV, Poopak EY, Leita K, et al. Vitrification versus slaw freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos［J］. J Assist Reprod Genet, 2009, 26(6):347-354.

［5］Zheng AY, Ding J, Gu B, et al. The effect on the embryo development potency and clinical outcome between vitrification and programmed freezing methods［J］. Reproduction & Contraception, 2013，33(1)：-. (in Chinese)

郑爱燕,丁洁, 顾斌,等. 玻璃化冷冻与程序化冷冻对胚胎发育潜能及临床结局的影响［J］. 生殖与避孕，2013，33(1)：16-20.

［6］Xing GB, Qiu Y, Meng JP. The impact of different embryo cryopreservation methods on the clinical outcome offreezing-thawed embryo transfer patients［J］. Maternal and Child Health Care of China, 2011, 26(15): 2301-2302. (in Chinese)

幸贵邦, 丘彦, 孟江萍. 不同胚胎冷冻方法对冻融胚胎移植患者临床结局的影响［J］. 中国妇幼保健, 2011, 26(15): 2301-2302.

［7］Balaban B, Urman B, Ata B, et al.A randomized controlled study of human day 3 embryo cryopreservation by slow freezing or vitrification: vitrification is associated with higher survival, metabolism and blastocyst formation［J］. Hum Reprod, 2008, 23(9):1976-1982.

［8］Lane M, Maybach JM, Gardner DK. Addition of ascorbate during cryopreservation stimulates subsequent embryo development［J］. Hum Reprod, 2002, 17(10):2686-2693.

［9］Sheehan CB, Lane M, Gardner DK. The CryoLoop facilitatesre-vitrification of embryos at four successive stages of development without impairing embryo growth［J］. Hum Reprod, 2006, 21(11):2978-2984.

［10］Giovanna F, Nicolas F, Anne S V，etal. A randomized controlled trial comparing two vitrification methods versus slow-freezing for cryopreservation of human cleavage stage embryos［J］. J Assist Reprod Genet, 2014, 31(4):241-247.

［11］Kolibianakis E M, Venetis C A, Tarlatzis B C. Cryopreservation of human embryos by vitrification or slow freezing: which one is better［J］.CurrOpin Obstet Gynecol, 2009, 21(3): 270-274.

［12］Wang YF, Yu ChM, Yang HY, et al. Comparison and analysis of the maternal and neonatal outcomes of programmed freezing and vitrification for cleavage stage embryo transfer［J］. Journal of Reproductive Medicine, 2014, 23(1):11-15. (in Chinese)

王宇峰，于春梅，杨海燕，等.程序化冷冻与玻璃化冷冻卵裂期胚胎移植的母婴结局对照分析［J］. 生殖医学杂志，2014, 23(1):11-15.

［13］Xue YM, Li K, Ge HSh, et al. Comparision of the clinical outcome of day 2 and day 3 embryo after cryopreservation with vitrification and programmed freezing［J］. Journal of Medical Research, 2011, 40(3):44-48. (in Chinese)

薛亚梅, 李坤, 葛红山, 等. 程序化与玻璃化冷冻保存人D2和D3胚胎的临床结局比较［J］. 医学研究杂志, 2011, 40(3):44-48.

［14］El-Toukhy T, Khalaf Y, Al-Darazi K, et al. blastomere loss on the outcome of frozen embryo replacement cycles［J］. Fertil Steril, 2003, 79(5):1106-1111.

［15］Archer J, Gook D, Edgar H. Blastocyst formation and cell numbers in human.frozen-thawed embryos following extended culture［J］. Hum Reprod, 2003, 18(8):1669-1673.

［16］Edgar D, Archer J, McBain J, et al. Embryonic factors affecting outcome from single cryopreserved embryo transfer［J］. Reprod Biomed Online, 2007, 14(6):718-723.

［17］Zheng X, Liu P, Chen G,et al. Viability of frozen-thawed human embryos with one-two blastomere lysis［J］. J Assist Reprod Genet, 2008, 25(7):281-285.

［18］Van Landuyt L, Van de Velde H, De Vos A, et al. Influence of cell loss after vitrification or slow-freezing on furtherin vitro development and implantation of human Day 3 embryos［J］. Hum Reprod, 2013, 28(11): 2943-2949.