Expression and localization of glucose transporter 1 and glucose transporter 2 under heat stress conditions

XI Hua-ming FAN Xiao-rui ZHANG Zhen LIANG Ya-jun HE Jun-ping*

doi:10.16098/j.issn.0529-1356.2017.04.013

PDF(2814 KB)

Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (4) : 445-451. DOI: 10.16098/j.issn.0529-1356.2017.04.013

Histology,Embryology and Developmental Biology

Expression and localization of glucose transporter 1 and glucose transporter 2 under heat stress conditions

XI Hua-ming FAN Xiao-rui ZHANG Zhen LIANG Ya-jun HE Jun-ping*

Author information +

History +

Abstract

Objective To investigate the expression and localization of glucose transporter 1 (GLUT1) and glucose transporter 2 (GLUT2) in adult boar testis under normal temperature and heat stress conditions. Methods Nine adult boars (Landrace) were randomly divided into three groups. The self-made thermo-controlled 42 ℃ blanket was used in local scrotal heating group (42 ℃ for 1 hour) (n=3). The boars of environmental heat stress group (n=3) were kept in a thermally-controlled hot house (37-40 ℃, 7 days, 3 hours per day). After the daily heat treatment, the boars were back to the normal temperature. Three boars were assigned as control (n=3) and kept in normal temperature house (21-25 ℃). After 6 hours (local scrotal heating group) and 24 hours (environmental heat stress group) of heat treatment, the boar testes were surgically harvested. The expression of GLUT1 and GLUT2 were detected in boar testes by using Real-time PCR, Western blotting and immunohistochemistry. Results The results of Real-time PCR and Western blotting showed that the GLUT1 protein and mRNA expression levels in the environmental heat stress group did not have significantly differences compared with control group. The GLUT1 protein and mRNA expression levels in the local scrotal heating group significantly increased compared with control group. In environmental heat stress group and local scrotal heating group, the expression levels of GLUT2 protein and mRNA significantly increased compared with control group. Immunohistochemical results showed that GLUT1 protein in seminiferous tubules was expressed in spermatocyte and round spermatid before and after heat treatment. In environmental heat stress group, the immunostaining of GLUT1 protein did not have significantly differences compared with control group. After local scrotal heating, the immunostaining of GLUT1 protein was deeper than control group, and the expression level of GLUT1 protein was increased. Before and after heat treatment, the GLUT2 protein in seminiferous tubules was expressed in germ cells and sertoli cells. The environmental heat stress and the local scrotal heating resulted in increase of GLUT2 expression and deeper immunostaining. Conclusion Glucose transporter GLUT1 and GLUT2 are expressed in the seminiferous tubules of boar testes. Environmental heat stress and local scrotal heating result in changes of GLUT1 and GLUT2 expression levels in the boar testis. The result suggests that GLUT1 and GLUT2 play important roles in boar spermatogenesis.

Key words

Heat stress / Glucose transporter 1 / Glucose transporter 2 / Testis / Western blotting / Immunohistochemistry / Boar

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

XI Hua-ming FAN Xiao-rui ZHANG Zhen LIANG Ya-jun HE Jun-ping. Expression and localization of glucose transporter 1 and glucose transporter 2 under heat stress conditions[J]. Acta Anatomica Sinica. 2017, 48(4): 445-451 https://doi.org/10.16098/j.issn.0529-1356.2017.04.013

References

［1］Roy VK, Krishna A. The expression pattern of the glucose transporter GLUT-5 in the testis during the spermatogenic cycle of the vespertilionid bat Scotophilus heathi ［J］. Gen Comp Endocr, 2013, 191(9):59.

［2］Gomez O, Romero A, Terrado J, et al. Differential expression of glucose transporter GLUT8 during mouse spermatogenesis ［J］. Reproduction, 2006, 131(1):63-70.

［3］Kim ST, Moley KH. The expression of GLUT8, GLUT9a, and GLUT9b in the mouse testis and sperm ［J］. Reprod Sci, 2007, 14(5):445-455.

［4］Davis JR. Metabolic aspects of spermatogenesis ［J］. Biol Reprod, 1969, Suppl 1:93-118.

［5］Wood IS, Trayhurn P. Glucose transporters (GLUT and SGLT): expanded families of sugar transport proteins ［J］. Br J Nutr, 2003, 89(1):3-9.

［6］Scheepers A, Joost HG, Schürmann A. The glucose transporter families SGLT and GLUT: molecular basis of normal and aberrant function ［J］. JPEN J Parenter Enteral Nutr, 2004, 28(5):364-371.

［7］Zhao FQ, Keating AF. Functional properties and genomics of glucose transporters ［J］. Curr Genomics, 2007, 8(2):113-128.

［8］Sun L, Zeng X, Yan C, et al. Crystal structure of a bacterial homologue of glucose transporters GLUT1-4 ［J］. Nature, 2012, 490(7420):361-366.

［9］Han J, Liang WM, Hong Y, et al. Expression of islet amyloid polypeptide and glucose transporter 2 in the islet β cell of rat during heroin withdrawal, detoxication and relapse ［J］. Acta Anatomica Sinica, 2013, 44(6):795-799.(in Chinese)

韩晶, 梁文妹, 洪艳, et al. 胰岛淀粉样多肽、葡萄糖转运蛋白2在海洛因戒断、脱毒和复吸大鼠胰岛β细胞中的表达［J］. 解剖学报, 2013, 44(6):795-799.

［10］Kokk K, Ver?j?nkorva E, Wu XK, et al. Immunohistochemical detection of glucose transporters class I subfamily in the mouse, rat and human testis ［J］. Medicina, 2004, 40(2):156-160.

［11］Kokk K, Ver?j?nkorva E, Wu XK, et al. Expression of insulin signaling transmitters and glucose transporters at the protein level in the rat testis ［J］. Ann N Y Acad Sci, 2007, 1095(1):262-273.

［12］Castillo J, Crespo D, Capilla E, et al. Evolutionary structural and functional conservation of an ortholog of the GLUT2 glucose transporter gene (SLC2A2) in zebrafish ［J］. Am J Physiol Regul Integr Comp Physiol, 2009, 297(5):R1570-1581.

［13］Hansen PJ. Effects of heat stress on mammalian reproduction ［J］. Philos Trans R Soc Lond B Biol Sci, 2009, 364(1534):3341-3350.

［14］Ivell R. Lifestyle impact and the biology of the human scrotum ［J］. Reprod Biol Endocrinol, 2007, 5(1):15.

［15］Setchell BP. The parkes lecture. Heat and the testis ［J］. J Reprod Fertil, 1998, 114(2):179-194.

［16］Gau BH, Chu IM, Huang MC, et al. Transcripts of enriched germ cells responding to heat shock as potential markers for porcine semen quality ［J］. Theriogenology, 2008, 69(6):758-766.

［17］Guo X, Chi S, Cong X, et al. Baicalin protects sertoli cells from heat stress-induced apoptosis via activation of the Fas/FasL pathway and Hsp72 expression ［J］. Reprod Toxicol, 2015, 57(1):196.

［18］Li XX, Chen SR, Shen B, et al. The heat-induced reversible change in the blood-testis barrier (BTB) is regulated by the androgen receptor (AR) via the partitioning-defective protein (Par) polarity complex in the mouse ［J］. Biol Reprod, 2001, 89(1):12.

［19］Salces-Ortiz J, Ramón M, González C, et al. Differences in the ovine HSP90AA1 gene expression rates caused by two linked polymorphisms at its promoter affect rams sperm DNA fragmentation under environmental heat stress conditions ［J］. PLoS One, 1932, 10(2):e0116360.

［20］Xun W, Shi L, Cao T, et al. Dual functions in response to heat stress and spermatogenesis: characterization of expression profile of small heat shock proteins 9 and 10 in goat testis ［J］. Biomed Res Int, 2015, 2015(10):1-8.

［21］Joost HG, Thorens B. The extended GLUT-family of sugar/polyol transport facilitators: nomenclature, sequence characteristics, and potential function of its novel members (review) ［J］. Mol Membr Biol, 2001, 18(4):247-256.

［22 Uldry M, Thorens B. The SLC2 family of facilitated hexose and polyol transporters ［J］. Pflugers Arch, 2004, 447(5):480-489.

［23］Farooqui SM, Al-Bagdadi F, O’Donnell JM, et al. Degenerative changes in spermatogonia are associated with loss of glucose transporter (Glut 3) in abdominal testis of surgically induced unilateral cryptorchidism in rats ［J］. Biochem Biophys Res Commun, 1997, 236(2):407-412.

［24］Fleeger JL, Vandemarka NL, Johnsona AD. The effect of carbon dioxide level and temperature on the in vitro metabolism of testis tissue ［J］. Comp Biochem Physiol, 1967, 23(2):475-482.