Impacts of silencing CD105 and Ki67 gene expression on the biological behavior of ovarian cancer OVCAR3 cells

GUO Hai-rong HE Shuai WANG Xiao-yan LIU Ping*

doi:10.16098/j.issn.0529-1356.2017.04.011

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (4) : 434-439. DOI: 10.16098/j.issn.0529-1356.2017.04.011

Cancer Biology

Impacts of silencing CD105 and Ki67 gene expression on the biological behavior of ovarian cancer OVCAR3 cells

GUO Hai-rong¹ HE Shuai²WANG Xiao-yan¹ LIU Ping^1*

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Abstract

Objective To investigate the effect of CD105 and Ki67 silence on ovarian cancer cell line OVCAR3 cells. Methods OVCAR3 cells were transfected with siRNA of CD105-siRNA ggroup, Ki67-siRNA group, CD105-siRNA+Ki67-siRNA group, negative control groups in vitro respectively. Cell proliferation, migration, invasion and apoptosis after transfection were analysed with MTT assay, wound-healing assay, Transwell assay and flow-cytometric. Results Compared with the negative control group, the expressions of mRNA and protein in CD105-siRNA group and Ki67-siRNA group were decreased significantly;Cell proliferation, migration, invasion ability were decreased significantly after transfection; apoptosis was increased. The most significant change was detected in CD105-siRNA+Ki67-siRNA group（P<0.01,P<0.05）. Conclusion CD105-siRNA and ki67-siRNA expression vector can inhibit the proliferation of human ovarian cancer OVCAR3 cells and reduce their cell migration and invasion, and induce apoptosis in tumor cells. The effect of the double gene silence is more obvious.

Key words

Ovarian cancer / CD105 / Ki67 / Proliferation / Migration / Western blotting / Human

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GUO Hai-rong HE Shuai WANG Xiao-yan LIU Ping. Impacts of silencing CD105 and Ki67 gene expression on the biological behavior of ovarian cancer OVCAR3 cells[J]. Acta Anatomica Sinica. 2017, 48(4): 434-439 https://doi.org/10.16098/j.issn.0529-1356.2017.04.011

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