Expression pattern of Kr&uuml;ppel-like factor 4 during adipogenic differentiation of porcine adipogenic mescenchymal stem cells

LI Xiu LI Fang-zheng HUAN Yan-jun GAO Xia JIANG Zhong-ling SONG Xue-xiong*

doi:10.16098/j.issn.0529-1356.2017.04.008

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (4) : 416-420. DOI: 10.16098/j.issn.0529-1356.2017.04.008

Expression pattern of Krüppel-like factor 4 during adipogenic differentiation of porcine adipogenic mescenchymal stem cells

LI Xiu LI Fang-zheng HUAN Yan-jun GAO Xia JIANG Zhong-ling SONG Xue-xiong*

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Abstract

Objective To investigate the expression pattern of Krüppel-like factor 4 (KLF4) during adipogenic differentiation of porcine adipogenic mescenchymal stem cells (AMSCs). Methods More than F3 generation of porcine AMSCs were used during adipogenic differentiation, the level of adipogenic differentiation was detected by oil red O staining extraction assay, the expression of KLF4 was analyzed by RT-PCR and Real-time PCR. Results During the porcine AMSCs adipogenic differentiation, the lipid droplets began to appear on 3 days after induced, and the rate of adipogenic differentiation was about 60% on 10 days after induced;During the day of 2, 4, 8, 12 and 16 induced, the expression of KLF4 was detected in each detect time by RT-PCR; Quantitative analysis of the KLF4 expression levels on the above days by Real-time PCR, showed 1.6657±0.2269, 2.6790±0.0524, 2.6293±0.0280, 2.3633±0.1568 and 2.6146±0.1575 folds high compared with the uninduced control group. These results suggested that the expression of KLF4 was significantly increased on the day 2 induced, the highest expression was on the day 4 induced, and remained high expression after then. Conclusion During porcine AMSCs adipogenic differentiation, KLF4 may play a continuous regulatory role from the early to later period of induction.

Key words

Adipogenic mescenchymal stem cell / Adipogenic differentiation / Krüppel-like facter 4 / Real-time PCR / Porcine

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LI Xiu LI Fang-zheng HUAN Yan-jun GAO Xia JIANG Zhong-ling SONG Xue-xiong. Expression pattern of Krüppel-like factor 4 during adipogenic differentiation of porcine adipogenic mescenchymal stem cells[J]. Acta Anatomica Sinica. 2017, 48(4): 416-420 https://doi.org/10.16098/j.issn.0529-1356.2017.04.008

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