Mouse induced pluripotent stem cell-derived cortical organoids and its biological characteristics

FAN Wen-juan WANG Qian SUN Yi-zheng WANG Lai DENG Jin-bo*

doi:10.16098/j.issn.0529-1356.2017.04.004

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (4) : 387-396. DOI: 10.16098/j.issn.0529-1356.2017.04.004

Neurobiology

Mouse induced pluripotent stem cell-derived cortical organoids and its biological characteristics

FAN Wen-juan WANG Qian SUN Yi-zheng WANG Lai DENG Jin-bo*

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Abstract

Objective To culture cerebral organoid from mouse induced pluripotent stem cells (iPSCs) and analyze its biological characteristics. Methods iPSCs were cultured into embryoid bodies (EB) through suspension cultures. EB were induced with some nervous growth factors and transfered to Matrigel droplet. After 1-2 months culture, cerebral organoids were formed. Immunolabeling, transmission electron microscopy, slice co-culture and DiO tracing were carried out to identify their biological characteristics. Results iPSCs-derived cerebral cortical organoids differentiated into neural precursor cells, nervous cells and glial cells. The cerebral organoid had cortical lamination, contained neuroepithelium, cortical plate and molecular layer and had the ability of neural regeneration and neural repair. Conclusion The cerebral organoid derived from mouse iPSCs l was cultured successfully, and the cultured cerebral organoid had similar biological characteristics with mammal cerebrum, including neural differentiation, cortical lamination, and especially neural regeneration and repair.

Key words

Induced pluripotent stem cells cells / Cerebral cortical organoid / Three dimensional culture / Neuroregeneration / Stem cell transplantation / Immunofluorescence / Mouse

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FAN Wen-juan WANG Qian SUN Yi-zheng WANG Lai DENG Jin-bo. Mouse induced pluripotent stem cell-derived cortical organoids and its biological characteristics[J]. Acta Anatomica Sinica. 2017, 48(4): 387-396 https://doi.org/10.16098/j.issn.0529-1356.2017.04.004

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