Effects of 6-hydroxydopamine on dopaminergic neurons in nigrostriatal system and on behavior of adult mice

XIE Ming-qi CHEN Zhi-chi QI Shuang-shuang WANG Tong-tong ZHANG Peng  HUANG Hou-ju ZHOU Peng CUI Huai-rui SUN Chen-you*

doi:10.16098/j.issn.0529-1356.2017.04.001

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (4) : 361-374. DOI: 10.16098/j.issn.0529-1356.2017.04.001

Neurobiology

Effects of 6-hydroxydopamine on dopaminergic neurons in nigrostriatal system and on behavior of adult mice

XIE Ming-qi^1,2 CHEN Zhi-chi^1,2 QI Shuang-shuang³ WANG Tong-tong^1,2 ZHANG Peng⁴ HUANG Hou-ju ^2,5 ZHOU Peng^1,2 CUI Huai-rui¹SUN Chen-you^{1, 2*}

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Abstract

Objective To evaluate the proliferation of endogenous neural progenitor cells (NPCs) and its effect on the recovery of injured nigrostiatal system following mesencephalic substantia nigra lesion. Methods The proliferation of NPCs derived from the surrounding regions of the lateral ventricle, the third ventricle (3V) and the cerebral aqueduct (Aq) as well as the midbrain were investigated using immunofluorescent staining on days 3-35 following 6-hydroxydopamine (6-OHDA) injection into the unilateral substantia nigra (SN) of the adult mice. In addition, the proliferation of the SN newborn cells and their differentiation into mature neurons or dopaminergic neurons were explored. Finally, behavioral changes of mice were examined by open field and rotarod tests(n=4-6,each group).
Results The 6-OHDA-induced loss of dopaminergic neurons in the SN significantly increased the numbers of NPCs from the subventricular zone in the surrounding regions of 3V and Aq, which was most obvious on day 7 following 6-OHDA injection. The number of SN new-born cells and new-generated dopaminergic neurons reached to the peak on day 21 following 6-OHDA injection, which possibly resulted in a partial restoration in the lesioned nigrastriatum system and behavioral performance of mice. Conclusion It might be an ideal strategy to deal with Parkinson’s disease by promoting the proliferation and differentiation of endogenous NPCs.

Key words

Parkinson’s disease

/ Substantia nigra / Neural progenitor cell / Dopaminergic neurons / 6-Hydroxydopamine / Immunofluorescence / Mouse

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XIE Ming-qi CHEN Zhi-chi QI Shuang-shuang WANG Tong-tong ZHANG Peng HUANG Hou-ju ZHOU Peng CUI Huai-rui SUN Chen-you. Effects of 6-hydroxydopamine on dopaminergic neurons in nigrostriatal system and on behavior of adult mice[J]. Acta Anatomica Sinica. 2017, 48(4): 361-374 https://doi.org/10.16098/j.issn.0529-1356.2017.04.001

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