Comparison of distribution characteristics of extracellular matrix components in the testis of the Tibetan sheep and the Small-tail Han sheep from Plateau

Ligang YUAN ZHANG Yong Cong Li CHENG Xu-dong LU Qi-zhong

doi:10.16098/j.issn.0529-1356.2017.02.011

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (2) : 179-186. DOI: 10.16098/j.issn.0529-1356.2017.02.011

Histology,Embryology and Developmental Biology

Comparison of distribution characteristics of extracellular matrix components in the testis of the Tibetan sheep and the Small-tail Han sheep from Plateau

Ligang YUANZHANG Yong²,Cong Li³,CHENG Xu-dong²,LU Qi-zhong²

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Abstract

Objective To compare the distribution and histochemical characteristics of extracellular matrix components in the testis between 4 Tibetan sheep and 5 small-tail Han sheep from plateau at the same age. Methods Histochemistry and transmission electron method were used to characterize the microstructure and ultrastructure of the two kinds sheep testis. Image-Pro Plus(IPP) statistics methods were used to identify the integrated absorbance for laminin(LN), type Ⅳ collagen(ColⅣ) and heparan sulfate proteoglycans(HSPG) distribution investigation by immunohistochemistry. Results The light microscopy observation showed that compared with the testis of the small-tail Han sheep from plateau, the collagen and reticular fibers were richer in interstitial of the Tibetan plateau sheep. The periodic acid-schiff(PAS) and Alcian blue-periodic acid schiff(AB-PAS) staining positive reactions were more obvious in interstitial basement and blood capillary of the Tibetan plateau sheep testis. Immunostaining analysis showed that the Col Ⅳ was present strongly in the seminiferous epithelium of both the Tibetan plateau sheep and the small-tail Han sheep. The expression of LN in the Sertoli cells and peritubular myoid cells of the Tibetan plateau sheep testis was weak but moderately positive in the Sertoli cells, the Leydig cells and peritubular myoid cells of the small-tail Han sheep testis. In the both kinds of sheep testis, the expression of HSPG was especially strong in the peritubular myoid cells but weak in the Sertoli cells and Leydig cells. The integrated absorbance of Col Ⅳ and LN expression was significantly lower in testis of the Tibetan sheep than the smalltail Han sheep (P<0.01）, but the average absorbance of HSPG expression was markedly higher than it (P<0.01). Electron micrographs of portion of the seminiferous tubule in the both of the testis showed elaborately developed tunicae propria with a well defined epithelial basal lamina and a distinct layer of typeⅠcollagen fibrils. The collagen fibrils were found around the interstitial blood capillaries and the abundant Lipid droplets in the Leydig cells of the small-tail Han sheep testis from plateau. Conclusion In plateau environment, the connective tissue in the tunicae propria and interstitial tissue of the Tibetan sheep testis is more abundant, and partly affect the development of the seminiferous epithelium. The AB-PAS positive reaction in interstitial basement and the wall of blood capillary in the Tibetan plateau sheep testis are closely related to the secretory function of the Leydig cells. The integrated absorbance of LN expression is significantly higher and HSPG is lower markedly in testis of the smalltail Han sheep testis, which is very important to the development degree of seminiferous epithelium.

Key words

Testis / Exreacellular matrix / Histochemistry / Immunohistochemistry / Tibetan plateau sheep / Small-tail Han sheep

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Ligang YUAN ZHANG Yong Cong Li CHENG Xu-dong LU Qi-zhong. Comparison of distribution characteristics of extracellular matrix components in the testis of the Tibetan sheep and the Small-tail Han sheep from Plateau[J]. Acta Anatomica Sinica. 2017, 48(2): 179-186 https://doi.org/10.16098/j.issn.0529-1356.2017.02.011

References

［1］Miqueloto CA, Zorn TM. Characterization and distribution of hyaluronan and the proteoglycans decorin, biglycan and perlecan in the developing embryonic mouse gonad.［J］.J Anat, 2007, 211(1):16-25.

［2］Siu MK，Cheng CY. Extracellular matrix and its role in spermatogenesis［J］. Adv Exp Med Biol, 2008, 636:74-91.

［3］Baert Y, Stukenborg JB, Landreh M, et al. Derivation and characterization of a cytocompatible scaffold from human testis［J］. Hum Reprod，2015,30(2):256-267.

［4］Yuan LG，Qu YL，Zhu JF, et al. Structure and distribution of extracellular matrix proteins in yak calves testis ［J］. Acta Anatomica Sinica，201546 (1):106-112. (in Chinese)

袁莉刚，曲亚玲，朱俊峰，等. 牦牛犊牛睾丸结构特征及细胞外基质相关蛋白的分布［J］.解剖学报， 2015,46 (1):106-112.

［5］Li C，Yuan LG，Qu YL, et al. The Histochemical study of extracellular matrix in plateau Tibetan sheep

epididymis［J］. Acta Veterinaria et Zootechnica Sinica, 2016,46 (4): 820-827. (in Chinese)

李聪，袁莉刚，曲亚玲，等.高原型藏绵羊附睾细胞外基质的组织化学研究［J］.畜牧兽医学报, 2016, 46 (4): 820-827.

［6］Xin JW, Mao XR, Ma ZhJ. Progress of studies on the genetic markers of the Tibetan sheep［J］. Journal of Qinghai University(Nature Science), 2007,25(1):78-82. (in Chinese)

信金伟，毛学荣，马志杰. 藏系绵羊遗传标记的研究进展［J］.青海大学学报(自然科学版), 2007,25(1):78-82.

［7］Li XL，Zhang ZL，Gong YF, et al. Study on m tDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP［J］.Hereditas, 2006,28(2):165-170.(in Chinese)

李祥龙, 张增利, 巩元芳, 等.我国主要地方绵羊品种mtDNA D-loop区PCR-RFLP研究［J］. 遗传, 2006,28(2):165-170.

［8］Hoflack G, Van Den Broeck W, Maes D, et al. Testicular dysfunction is responsible for low sperm quality in Belgian Blue bulls［J］. Theriogenology, 2008,69(3): 323-332.

［9］Fakoya FA. Reticulin fibres in the tunica albuginea and peritubular tissue of seminiferous tubules of adult male Wistar rats［J］.Acta Histochem, 2002,104(3):279-283.

［10］Paick JS, Park K, Kim SW, et al. Increased expression of hypoxia-inducible factor-1α and connective tissue growth factor accompanied by fibrosis in the rat testis of varicocele.［J］. Actas Urol Esp, 2012,36(5):282-288.

［11］Adrid E, Reyes JG, Hernández B, et al. Effect of normobaric hypoxia on the testis in a murine model ［J］.Andrologia, 2013,45(5):332-338.

［12］Yuan LG，Zhu JF，Gu LF, et al. The histologic characters of testis in aging yak ［J］. Acta Veterinaria et Zootechnica Sinica, 2015, 46 (9):1656-1662. (in Chinese)

袁莉刚，朱俊峰，谷来凤，等. 老龄牦牛睾丸细胞外基质相关蛋白的分布特征［J］. 畜牧兽医学报，2015， 46(9)：1656-1662.

［13］Pop OT, Cotoi CG, Ple??塂ea IE, et al. Histological and ultrastructural analysis of the seminiferous tubule wall in ageing testis ［J］.Rom J Morphol Embryol, 2011,52(1): 241-248.

［14］Moore BC, Hamlin HJ, Botteri NL, et al. Posthatching development of alligator mississippiensis ovary and testis ［J］. J Morphol, 2010,271(5):580-595.

［15］Abd-Elmaksoud A，Sayed-Ahmed A，Kassab M, et al. Histochemical mapping of glycoconjugates in the testis of the one humped camel(Camelus dromedarius) during rutting and non-rutting seasons. ［J］. Acta Histochem,2008,110(2):124-133.

［16］Osman DI，Moniem KA，Tingari MD. Studies on the testis of the camel (Camelus dromedarius). III. Histochemical observations ［J］. Histochem J, 1976,8(6):579-590.

［17］Azevedo MH, Paula TA, Balarini MK, et al. Organization and quantification of the elements in the intertubular space in the adult jaguar testis (Panthera onca, LINNAEUS, 1758) ［J］.Micron, 2008,39(8):1166-1170.

［18］Beltrán-Frutos E, Seco-Rovira V, Ferrer C, et al. Changes in testicular interstitial connective tissue of hamsters (Mesocricetus auratus) during ageing and after exposure to short photoperiod ［J］.Reprod Domest Anim, 2016,51(1):47-53.

［19］Brown BN, Badylak SF. Extracellular matrix as an inductive scaffold for functional tissue reconstruction ［J］. Transl Res, 2014,163(4):268-285.

［20］Glattauer V, Irving-Rodgers HF, Rodgers RJ, et al. Examination of basement membrane components associated with the bovine seminiferous tubule basal lamina ［J］.Reprod Fertil , 2007,19(3):473-481.

［21］Anbalagan M, Rao AJ. Collagen IV-mediated signalling is involved in progenitor Leydig cell proliferation［J］.Biomed Online, 2004,9(4):391-403.

［22］Diaz ES，Pellizzari E，Casanova M, et al. Type IV collagen induces down-regulation of steroidogenic response to gonadotropins in adult rat Leydig cells involving mitogen-activated protein kinase ［J］.Mol Reprod Dev,2005,72(2):208-215.

［23］Abd-Elmaksoud A. Comparative expression of laminin and smooth muscle actin in the testis and epididymis of poultry and rabbit ［J］.J Mol Histol，2009,40(56)：407-416.

［24］Lustig L, Denduchis B, Ponzio R, et al. Passive immunization with anti-laminin immunoglobulin G modifies the integrity of the seminiferous epithelium and induces arrest of spermatogenesis in the guinea pig ［J］.Biol Reprod, 2000,62(6):1505-1514.

［25］Poulain FE, Yost HJ. Heparan sulfate proteoglycans: a sugar code for vertebrate development ［J］ ? Development, 2015,142(20):3456-3467.

［26］Jiang DY，Fu XB，Sheng ZhY. The diversity of structure and function of heparin sulfate proteoglycans via modification of some relative enzymes ［J］.Chinese Journal of Pathophysiology, 2005,21(5):1020-1025.(in Chinese)

姜笃银，付小兵，盛志勇.硫酸乙酰肝素糖蛋白的结构功能多样性与相关修饰酶群作用［J］.中国病理生理杂志, 2005,21(5)：1020-1025.

［27］Mcfarlane JR，Laslett A，Kretser DM, et al. Evidence that heparin binding autocrine factors modulate testosterone production by the adult rat Leydig cell ［J］. Mol Cell Endocrinol, 1996,118(1-2)：57-63.

［28］Brucato S, Bocquet J, Villers C. Cell surface heparan sulfate proteoglycans: target and partners of the basic fibroblast growth factor in rat Sertoli cells ［J］.Eur J Biochem, 2002,269(2):502-511.