Objective To establish a radioresistant model of non-small-cell cancer A549, and to provide the experimental basis for further researchonradioresistance.Methods The cell number and the dose of radiation therapy were confirmed. The intermittent radiation were used to induce the radioresistant cell model. The morphology of cells were observed with an inverted phase contrast microscope. Colony formation was used to identify the radioresistance of the RA549 cell.Results RA549 cells were longer and bigger than A549 cells; surviving fraction of RA549 was significantly increased than A549. Conclusion Intermittent radiation can successfully induce the radioresistant cell model of lung cancer cells.