Novel protein C7orf42 promotes rat hepatocyte BRL-3A proliferation

ZHANG Chun-yan CHANG Cui-fang ZHANG Shi-fu MA Ji ZHANG Fu-chun XU Cun-shuan*

doi:10.16098/j.issn.0529-1356.2017.02.005

PDF(415 KB)

Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (2) : 150-155. DOI: 10.16098/j.issn.0529-1356.2017.02.005

Cell and Molecules Biology

Novel protein C7orf42 promotes rat hepatocyte BRL-3A proliferation

ZHANG Chun-yan^1,2 CHANG Cui-fang²ZHANG Shi-fu²MA Ji¹ZHANG Fu-chun¹XU Cun-shuan^2*

Author information +

History +

Abstract

Objective To explore the effect of C7orf42 on cell proliferation of rat hepatocyte line BRL-3A in vitro. Methods The expression of C7orf42 was knocked down by siRNA, and MTT and EdU assay were used to discover the effect of C7orf42 on cell proliferation at 48 hours after transfection. Flow cytometry was used to observe the effect of cell cycle progression. Real-tme PCR and Western blotting were used to detect the changes in the expression of cell proliferation-associated gene. Results MTT results showed that the cell viability of the interference group (C7BRL-siR3) was significantly lower than that of the negative control group (NC) at 48 hours after transfection (P<0.05). Meanwhile, the percentage of EdUp-labeling cells was also significantly decreased (P<0.05). At the same time, the flow cytometry results showed that the number of cells in division phase (S+G₂/M) of the interference group was significantly reduced in parallel (P<0.05). Further, the interference group down-regulated the expression levels of cell proliferation-related genes and proteins of JUN, MYC, CCND1 and CCNA2. Conclusion C7orf42 may promote cell proliferation via regulating the expression of JUN, MYC, CCND1 and CCNA2 in rat hepatocyte line BRL-3A.

Key words

Novel protein / C7orf42 / Hepatocyte line BRL-3A / Small interfering RNA / Cell proliferation / Western blotting / Rat

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

ZHANG Chun-yan CHANG Cui-fang ZHANG Shi-fu MA Ji ZHANG Fu-chun XU Cun-shuan. Novel protein C7orf42 promotes rat hepatocyte BRL-3A proliferation[J]. Acta Anatomica Sinica. 2017, 48(2): 150-155 https://doi.org/10.16098/j.issn.0529-1356.2017.02.005

References

［1］Fausto N. Liver regeneration ［J］. J Hepatol, 2000, 32(1): 19-31.

［2］Jiang WW, Hao XX, Guo YQ, et al. Caspase signaling pathway regulates hepatocyte apoptosis in rat liver regeneration ［J］. Acta Anatomica Sinica, 2012, 43(3):359-365. (in Chinese)

蒋文文，郝晓霞，郭雅琼，等，Caspase信号通路调控大鼠再生肝肝细胞的凋亡［J］.解剖学报，2012,43(3):359-365.

［3］Michalopoulos GK, Defrances MC. Liver regenertation ［J］. Science, 1997, 276(5309): 60-66.

［4］Higgins GM, Anderson RM. Experimental pathology of the liver: restoration of the liver of the white rat following partial surgical removal ［J］. Arch Pathol, 1931, 12(2):186-202.

［5］Berasain C, García-Trevijano ER, Castillo J, et al. Amphiregulin: an early trigger of liver regeneration in mice ［J］. Gastroenterology, 2005, 128(2): 424-432.

［6］Perugorria MJ, Latasa MU, Nicou A, et al. The epidermal growth factor receptor ligand amphiregulin participates in the development of mouse liver fibrosis ［J］. Hepatology, 2008, 48(4): 1251-1261.

［7］Conway-Campbell BL, Wooh JW, Brooks AJ, et al. Nuclear targeting of the growth hormone receptor results in dysregulation of cell proliferation and tumorigenesis ［J］. Proc Natl Acad Sci USA, 2007, 104(33): 13331-13336.

［8］B?hm F, Khler UA, Speicher T, et al. Regulation of liver regeneration by growth factors and cytokines ［J］. EMBO Mol Med, 2010, 2(8): 294-305.

［9］Datta SR, Dudek H, Tao X, et al. AKT phosphorylation of BAD couples survival signals to the cell-intrinsic death mathinery ［J］. Cell, 1997, 91(2):231-241.

［10］Kalimuthu S, Se-Kwon K. Cell survival and apoptosis signaling as therapeutic target for canner: marine bioactive compounds ［J］. Int J Mol Sci, 2013, 14(2): 2334-2354.

［11］Kountouras J, Boura P, Lygidakis NJ. Liver regeneration after hepatectomy ［J］. Hepatogastroenterology, 2001, 48(38): 556-562.

［12］Xing XK, Wu HY, Lin JT, et al. Effect of chemokine 2 on the formation of lipid on liver regeneration ［J］. Acta Anatomica Sinica, 2016, 47(5):697-702. (in Chinese)

邢雪琨，武红艳，林俊堂，等，趋化因子2促进肝再生中脂肪的形成［J］. Acta Anatomica Sinica, 2016, 47(5): 697-702.

［13］Alcorn JA, Feitelberg SP, Brenner DA. Transient induction of c-jun during hepatic regeneration ［J］. Hepatology, 1990, 11(6): 909-915.

［14］Morello D. Differential regulation and expression of jun, c-fos and c-myc proto- oncogenes during mouse liver regeneration and after inhibition of protein synthesis ［J］. Oncogene, 1990, 5(10): 1511-1519.

［15］Miquet JG, Freund T, Martinez CS, et al. Hepatocellular alterations and dysregulation of oncogenic pathways in the liver of transgenic mice overexpressing growth hormone［J］. Cell Cycle, 2013, 12(7): 1042-1057.

［16］Behrens A, Sibilia M, David JP, et al. Impaired postnatal hepatocyte proliferation and liver regeneration in mice lacking c-jun in the liver ［J］. EMBO J, 2002, 21(7): 1782-1790.

［17］Riehle KJ, Campbell JS, McMahan RS, et al. Regulation of liver regeneration and hepatocarcinogenesis by suppressor of cytokine signaling 3［J］. J Exp Med, 2008, 205(1): 91-103.

［18］Haga S, Ogawa W, Inoue H, et al. Compensatory recovery of liver mass by Akt-mediated hepatocellular hypertrophy in liver-specific STAT3-deficient mice ［J］. J Hepatol, 2005, 43(5): 799-807.

［19］Albanese C, Johnson J, Watanabe G, et al. Transforming p21ras mutants and c-Ets-2 activate the cyclin D1 promoter through distinguishable regions ［J］. J Biol Chemi, 1995, 270(40): 23589-23597.

［20］Nelsen CJ, Rickheim DG, Timchenko NA, et al. Transient expression of cyclin D1 is sufficient to promote hepatocyte replication and liver growth in vivo ［J］. Cancer Res, 2001, 61(23): 8564-8568.

［21］Rickheim DG, Nelsen CJ, Fassett JT, et al. Differential regulation of cyclins D1 and D3 in hepatocyte proliferation ［J］. Hepatology, 2002, 36(1): 30-38.

［22］Ledda-Columbano GM, Pibiri M, Molotzu F,et al. Induction of hepatocyte proliferation by retinoic acid［J］. Carcinogenesis, 2004, 25(11):2061-2066.

［23］Chen YX, Zhang XR, Xie WF, et al. Effects of taurine on proliferation and apoptosis of hepatic stellate cells in vitro ［J］. Hepatobiliary Pancreat Dis Int, 2004, 3(1):106-109.

［24］Hsu MK, Qiao L, Ho V, et al. Ethanol reduces p38 kinase activation and cyclin D1 protein expression after partial hepatectomy in rats ［J］. J Hepatol, 2006, 44(2): 375-382.

［25］Hanse EA, Nelsen CJ, Goggin MM, et al. Cdk2 plays a critical role in hepatocyte cell cycle progression and survival in the setting of cyclin D1 expression in vivo ［J］. Cell Cycle, 2009, 8(17): 2802-2809.

［26］Garnier D, Loyer P, Ribault C, et al. Cyclin-dependent kinase 1 plays a critical role in DNA replication control during rat liver regeneration ［J］. Hepatology, 2009, 50(6): 1946-1956.