Effect of cryopreservation on the expression of sperm surface protein PH-20 and ratio of apoptosis in normal birth and infertility

MA Xiao-ping GAO Xiao-qin* ZHOU Hua WANG Jun

doi:10.16098/j.issn.0529-1356.2017.01.015

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (1) : 87-91. DOI: 10.16098/j.issn.0529-1356.2017.01.015

Histology,Embryology and Developmental Biology

Effect of cryopreservation on the expression of sperm surface protein PH-20 and ratio of apoptosis in normal birth and infertility

MA Xiao-ping ^1,2 GAO Xiao-qin^2* ZHOU Hua³WANG Jun⁴

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Abstract

Objective To investigate the effect of cryopreservation on the expression of sperm surface protein PH-20 and the sperm apoptosis. Methods Semen samples were obtained from fertile men (n=14, group A) and infertile men (n=20, group B). Western blotting was used to detect the PH-20 protein expression in human spermatozoa.The localization of this protein on human spermatozoa was determined by indirect immunofluorescent staining using PH-20 antibody. The sperm apoptosis was examined by terminal deoxynucleotidyl transferase(TdT) mediated deoxyuridine triphophate-biotin nick end labeling(TUNEL). Results After cryopreservation, the level of PH-20 protein expression was significantly lower in both group A and B than that of fresh sperm (P<0.05). The percentage of PH-20 positive rate was significantly lower in both group A and B than that of fresh sperm (P<0.05). The ratio of sperm apoptosis was not significantly different in thawed group A than that of fresh sperm (P>0.05). The ratio of sperm apoptosis was significantly lower in thawed group B than that of fresh sperm (P<0.05).
Conclusion Cryopreservation caused significant reduction of PH-20 protein expression, the percentage of PH-20 positive rate in human sperm. But the cryopreservation had no significant influence on normal fertility of sperm apoptosis.

Key words

Sperm / Cryopreservation / PH-20 / Western blotting / Human

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MA Xiao-ping GAO Xiao-qin ZHOU Hua WANG Jun. Effect of cryopreservation on the expression of sperm surface protein PH-20 and ratio of apoptosis in normal birth and infertility[J]. Acta Anatomica Sinica. 2017, 48(1): 87-91 https://doi.org/10.16098/j.issn.0529-1356.2017.01.015

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