Effect of Heshouwuyin on protein expression of steroidogenic acute regulatory protein and cytochrome P450 cholesterol side chain cleavage enzyme in Leydig cells of rats

WANG Jian-mingSUN Jing QI Feng SONG Qing-liang NIU Si-yun* HUI Chen-hong XU Tian-rong HE Fei

doi:10.16098/j.issn.0529-1356.2017.01.006

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Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (1) : 30-36. DOI: 10.16098/j.issn.0529-1356.2017.01.006

Effect of Heshouwuyin on protein expression of steroidogenic acute regulatory protein and cytochrome P450 cholesterol side chain cleavage enzyme in Leydig cells of rats

WANG Jian-ming ^1,2SUN Jing¹QI Feng³ SONG Qing-liang¹NIU Si-yun ^1* HUI Chen-hong¹XU Tian-rong¹ HE Fei¹

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Abstract

Objective To explore the effect of Heshouwuyin on the steroidogenic acute regulatory protein (StAR) and cytochrome P450 cholesterol side chain cleavage enzyme(P450scc) protein expression in Leydig cells of rats. Methods Juvenile male Wistar rat,10-20 days old, were used to isolate the Leydig cells and cultured, and then collected them. The oxidative damage technique was used to establish Leydig cell aging model. This study had a Leydig aging model, Heshouwuyin and Shouwu pill treatments, and normal and Heshouwuyin control group. Results The intensities of immunohistichemical staining for StAR and P450scc were significantly lower in the Leydig aging model group than those in the normal group and Heshouwuyin control group (P<0.01), and were significantly higher in the Heshouwuyin and Shouwu pill group than that in the aging model group (P<0.05). Heshouwuyin group was better than Shouwu pill group. Conclusion Heshouwuyin can increase the protein expression of StAR and P450scc in Leydig cells, improve the synthesis of testosterone, thus delaying the senescence of Leydig cells.

Key words

Heshouwuyin / Testicular Leydig cells / Immunohistichemistudy / Western blotting / StAR / P450scc / Rat

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WANG Jian-mingSUN Jing QI Feng SONG Qing-liang NIU Si-yun HUI Chen-hong XU Tian-rong HE Fei. Effect of Heshouwuyin on protein expression of steroidogenic acute regulatory protein and cytochrome P450 cholesterol side chain cleavage enzyme in Leydig cells of rats[J]. Acta Anatomica Sinica. 2017, 48(1): 30-36 https://doi.org/10.16098/j.issn.0529-1356.2017.01.006

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