Distribution of cardiac telocytes in the aged rat heart

XU Sha-sha LIAO Zhao-fu CAI Dong-qing*

doi:10.16098/j.issn.0529-1356.2016.06.014

Acta Anatomica Sinica ›› 2016, Vol. 47 ›› Issue (6) : 802-806. DOI: 10.16098/j.issn.0529-1356.2016.06.014

Histology,Embryology and Developmental Biology

Distribution of cardiac telocytes in the aged rat heart

XU Sha-sha ^1,2,3 LIAO Zhao-fu ^1,2,3 CAI Dong-qing ^1,2,3*

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Abstract

Objective To observe the morphology and distribution of cardiac Telocytes (CTs) in the aged rat heart and the difference compared with young rat heart. Methods The transmission electron microscopy combining bio-image analysis and immunofluorescence staining were applied to analyze the morphology and distribution of CTs in the atrium, medium and apex part of 24-month-old rat heart semiquantitatively. Results A lot of cells with unique morphology of CTs were found in interstitial tissue of cardiac myoctyes and microvessels in the atrium, medium and apex part. The morphology parameters of CTs were similar in the three representative dimensions of heart. The difference is not statistic significance. The immunofluorence staining documented that aged CTs as well as young CTs expressed c-Kit and CD34. In aged heart, the CT density in the atrium part（47.65±4.01 cells/mm²）was significantly higher than that of the apex part (33.68±2.53 cells/mm²) and the medium part(26.49±2.11 cells/mm²) (P<0.05). In addition, it was found that the distributional pattern of CTs in the three representative dimensions of aged heart was similar to that of young heart. However, the CT density in the three representative dimensions of aged heart is significantly higher than that of the parallel areas of young heart (P<0.05). Conclusion CTs are found in the aged rat heart. The CTs density of the atrium part is significantly higher than that of the apex part and the medium part in aged heart. In addition, the distributional pattern of CTs in aged heart is similar to young heart. However, the CTs density of aged heart in atrium, medium and apex part is significantly larger than that of parallel areas of young heart respectively.

Key words

Cardiac / Telocyte / Myocardium / Aging / Immunofluorescence / Rat

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XU Sha-sha LIAO Zhao-fu CAI Dong-qing. Distribution of cardiac telocytes in the aged rat heart[J]. Acta Anatomica Sinica. 2016, 47(6): 802-806 https://doi.org/10.16098/j.issn.0529-1356.2016.06.014

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