Change of learning and memory ability in neonatal rats with hypoxic-ischemic white matter injury

LIN Ling ZHANG Geng LIN Qiao-mei LIN Qing*

doi:10.16098/j.issn.0529-1356.2016.06.003

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Acta Anatomica Sinica ›› 2016, Vol. 47 ›› Issue (6) : 738-743. DOI: 10.16098/j.issn.0529-1356.2016.06.003

Neurobiology

Change of learning and memory ability in neonatal rats with hypoxic-ischemic white matter injury

LIN Ling ZHANG Geng LIN Qiao-mei LIN Qing*

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Abstract

Objective To explore changes of the learning and memory ability as well as the myelin basic protein (MBP) expression during various growth periods in neonatal rats with hypoxic-ischemic white matter injury (WMI). Methods New born 4-day-old SD rats were randomly divided into a control group and a hypoxicischemic WMI group. The histology of rat brain tissues was observed by HE staining at 4w after operation. The neurobehavior and the ability of learning and memory were investigated at 4weeks, 8weeks and 12weeks after operation. The distribution and expression of MBP protein were tested by immunofluorescent histochemical staining method and western blotting. Results The hypoxic-ischemic white matter injury was showed by HE staining in WMI group. The weaker ability of neurobehavior and learning and memory were displayed in WMI group compared with the control group (P＜0.01). The expression of MBP was significantly decreased in the WMI group compared with the control group（P＜0.01）. Conclusion The ability of learning and memory was weakened and the expression of MBP protein was decreased in new born 4-day-old SD rats with hypoxic-ischemic white matter injury. The weaker ability of learning and memory may be resulted from the decreased expression of MBP protein.

Key words

White matter injury / Learning / Memory / Myelin basic protein / Immunofluorescence / Western blotting / Rat

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LIN Ling ZHANG Geng LIN Qiao-mei LIN Qing. Change of learning and memory ability in neonatal rats with hypoxic-ischemic white matter injury[J]. Acta Anatomica Sinica. 2016, 47(6): 738-743 https://doi.org/10.16098/j.issn.0529-1356.2016.06.003

References

［1］Hou XL, Fen Q, Tang ZZh,et al. The opinion of expert group on diagnosis, prevention and management in premature infants with white matter injury［J］. Chinese Journal of Neonatology, 2015, 30(3): 175-177.(in Chinese)

侯新琳，冯琪，汤泽中，等.早产儿脑白质损伤诊断、防治与综合管理的专家组意见［J］.中国新生儿科杂志，2015, 30(3): 175-177.

［2］Deng W. Neurobiology of injury to the developing brain［J］. Nat Rev Neurol, 2010, 6(6): 328-336.

［3］Munck P, Haataja L, Maunu J, et al. Cognitive outcome at years of age in finnish infants with very low birth weight born between 2001 and 2006［J］. Acta Paediatr, 2010, 99(3): 359-366.

［4］Petersson KH, Pinar H, Stopa EG, et al. White matter injury after cerebral ischemia in ovine fetuses［J］. Pediatr Res, 2002, 51(6): 768-776.

［5］Liu XB, Shen Y, Plane JM, et al. Vulnerability of premyelinating oligodendrocytes to white-matter damage in neonatal brain injury［J］. Neurosci Bull, 2013, 29 (2): 229-238.

［6］Volpe JJ. Systemic inflammation, oligodendroglial maturation, and the encephalopathy of prematurity［J］. Ann Neurol, 2011, 70(4): 525-529.

［7］Hagen MW, Riddle A, McClendon E, et al. Role of recurrent hypoxia-ischemia in preterm white matter injury severity［J］. PLoS One, 2014, 9(11): el12800.

［8］Back SA, Riddle A, McClure MM. Maturation-dependent vulnerability of perinatal white matter in premature birth［J］. Stroke, 2007, 38(2 Suppl): 724-730.

［9］Back SA, Han BH, Luo NL, et al. Selective vulnerability of late oligodendrocyte progenitors to hypoxia-ischemia［J］. Neurosci, 2002, 22(2): 455-463.

［10］Ikeda T, Mishima K, Yoshikawa T, et al. Selective and long-term learning impairment following neonatal hypoxic-ischemic brain insult in rats［J］. Behav Brain Res, 2001, 118(1): 17-25.

［11］Mishima K, Ikeda T, Aoo N, et al. Hypoxia-ischemic insult in neonatal rats induced slowly progressive brain damage related to memory impairment［J］. Neuroscience Letters, 2005, 376(3): 194-199.

［12］Fields RD.Change in the brain’s white matter［J］. Science, 2010, 330(6005): 768-769.

［13］Back SA, Miller SP. Brain injury in premature neonates: a primary cerebral dysmaturation disorder ［J］? Ann Neurol, 2014, 75(4): 469-486.

［14］Buser JR, Maire J, Riddle A, et al. Arrested preoligodendrocyte maturation contributes to myelination failure in premature infants［J］. Ann Neurol, 2012, 71(1): 93-109.

［15］Riddle A, Dean J, Buser JR, et al. Histopathological correlates of magnetic resonance imaging-defined chronic perinatal white matter injury［J］. Ann Neurol, 2011, 70(3): 493-507.

［16］Yamazaki Y, Fujiwara H, Kaneko K, et al. Short and long-term functional plasticity of white Matter induced by oligodendrocyte depolarization in the hippocampus［J］. Glia, 2014, 62(8): 1299-1312.