Objective To explore the variation of sarcomere skelemin ɑ-actinin and Myomesin-1 during the left ventricular wall re-modeling in the rats with renal hypertension. Methods The animal model was prepared by bilateral renal artery stenosis, and renal artery was freed in the control group. Forty rats were randomly divided into sham operation group, two weeks group, five weeks group and seven weeks group after operation. The model quality of hypertensive rats were assessed by noninvasive blood pressure analysis tester and the indexes of left ventricular weight (LVW/BW). The immunohistochemistry and Western blotting was used to detect the expression of ɑ-actinin and Myomesin-1 in the ventricle muscle tissues, and microscope image analysis and statistical processing were carried out. Results ɑ-actinin and Myomesin-1 were expressed in myocardium and showed diffusion distribution during hypertensive. The results of the immunohistochemistry and Western blotting showed that the expression of ɑ-actinin and Myomesin-1 increased significantly at 2 weeks group after operation compared with the control group（P<0.01）, and declined significantly than control group in the process of hypertensive（P<0.01）. In addition, the width of positive expression exhibited a trend from declining to rising, and showed significance difference between neighboring groups（P<0.05）. Conclusion ɑ-actinin and Myomesin-1 play a role in the process of myocardial hypertrophy, suggesting that the level of blood pressure is associated with the sarcomere contractility.