Expression of sonic hedgehog and islet-1 in morphogenesis of respiratory system in mouse embryo

CAI Yu-jin LI Hui-chao ZHANG Hong-quan JING Ya* YANG Yan-ping CUI Hui-lin

doi:10.16098/j.issn.0529-1356.2016.05.018

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Acta Anatomica Sinica ›› 2016, Vol. 47 ›› Issue (5) : 678-682. DOI: 10.16098/j.issn.0529-1356.2016.05.018

Histology,Embryology and Developmental Biology

Expression of sonic hedgehog and islet-1 in morphogenesis of respiratory system in mouse embryo

CAI Yu-jin¹LI Hui-chao²ZHANG Hong-quan³ JING Ya ^1* YANG Yan-ping¹ CUI Hui-lin¹

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Abstract

Objective To explore the relationship of the early morphogenesis of respiratory system with the expression of islet-1(ISL1) and sonic hedgehog(Shh)-patched 1(Ptc1)-smoothened(Smo) pathway.Methods Serial sections of three mouse embryos each day from embryonic day(ED) 9.0 to ED 13 were stained immunohistochemically with antibodies against Shh, Ptcl, Smo, ISL1 and α-smooth muscle actin (α-SMA).Results At ED10, Shh was detected in the endodermal epithelium ventral to the foregut. From ED11 to ED12, Ptc1 was present in epithelial cells of both the trachea, bronchies and branching of lung buds. At ED13, Ptc1 expression was restricted to epithelial cells of airway in lung. At ED9, ISL1 staining of epithelial cells was observed in endoderm ventral to the foregut. From ED11 to ED12, ISL1 staining was observed in epithelial and mesenchyme cells surrounding ventral to the trachea and lateral to the bronchies, where colocalization of ISL1 with Smo was detected. At ED13, ISL1 staining was reduced, while little present in the distal airway of the developing lung. From ED12 to ED13, α-SMA positive smooth muscle cells were detected in mesenchyme adjacent closely to the dorsal wall of trachea and between bronchies where Ptc1 staining was negative in the epithelial. The distribution pattern of ISL1 positive cells and α-SMA positive cells in mesenchyme around trachea and bronchies was in dorsal-ventral or inner-lateral direction.Conclusion ISL1 may play an important role in normal morphogenesis of the trachea and lung buds in coordination with members of Shh-Ptc1-Smo pathway.

Key words

Sonic hedgehog / Islet-1 Respiratory system｜ Embryo｜ Immunohistochemistry｜ Mouse

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CAI Yu-jin LI Hui-chao ZHANG Hong-quan JING Ya YANG Yan-ping CUI Hui-lin. Expression of sonic hedgehog and islet-1 in morphogenesis of respiratory system in mouse embryo[J]. Acta Anatomica Sinica. 2016, 47(5): 678-682 https://doi.org/10.16098/j.issn.0529-1356.2016.05.018

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