FRMD8 inhibits amplification and migration of lung cancer cells

HU Jin-xia ZHAO Wei LI You-jie ZHAN Jun* XIE Shu-yang*

doi:10.16098/j.issn.0529-1356.2016.05.010

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Acta Anatomica Sinica ›› 2016, Vol. 47 ›› Issue (5) : 638-644. DOI: 10.16098/j.issn.0529-1356.2016.05.010

Cancer Biology

FRMD8 inhibits amplification and migration of lung cancer cells

HU Jin-xia¹ ZHAO Wei¹ LI You-jie¹ ZHAN Jun^2*XIE Shu-yang^1*

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Abstract

Objective To investigate the role of FERM domain-containing protein 8 （FRMD8）in lung cancer progression, and explore the mechanism. Methods Western blotting was used to detect expression of FRMD8 in 14 pairs of matched non-involved lung tissue and lung cancer tissues; Colony formation assay and MTT assay were performed for detecting cell proliferation after A549 cells were transfected with siRNAs. Flow cytometry/FACS was applied to detect apoptosis of A549 cells after FRMD8 being up-regulated, Expression of apoptosis-related proteins was evaluated by Western blotting following FRMD8 was up-regulated. A549 cells were transiently transfected with siRNAs or FRMD8 expression vectors, then Transwell migration assay was carried out to test the influence of FRMD8 on cell migration. Results 1. Compared with normal tissues, FRMD8 expression in lung cancer tissues was down-regulated significantly(P＜0.05); 2. Colony formation and MTT assay showed that inhibition of FRMD8 expression by siRNAs promoted proliferation of A549 cells; 3.Over-expression of FRMD8 induced apoptosis and regulated expression of apoptosis-related proteins: Caspase-3, Caspase-8 and Bax were down-regulated, and cleaved Caspase-3, Caspase-8 and bcl-2, p53 were up-regulated; 4. Down-regulation of FRMD8 promoted migration（P＜0.01）. Over-expression of FRMD8 repressed migration of A549 cells（P＜0.001）.Conclusion FRMD8 represses lung cancer progression and may act as a tumor repressor.

Key words

FRMD8 / Lung cancer / Migration / Western blotting / Human

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HU Jin-xia ZHAO Wei LI You-jie ZHAN Jun XIE Shu-yang. FRMD8 inhibits amplification and migration of lung cancer cells[J]. Acta Anatomica Sinica. 2016, 47(5): 638-644 https://doi.org/10.16098/j.issn.0529-1356.2016.05.010

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