Morphology and protein labeling of telocytes in cardiac neonatal rats

GAN Li CHANG Yu-qiao ZHANG San-lin NIU Yan-fei YU Xia GUO Zhi-kun*

doi:10.16098/j.issn.0529-1356.2016.05.009

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Acta Anatomica Sinica ›› 2016, Vol. 47 ›› Issue (5) : 633-637. DOI: 10.16098/j.issn.0529-1356.2016.05.009

Cell and Molecules Biology

Morphology and protein labeling of telocytes in cardiac neonatal rats

GAN Li¹ CHANG Yu-qiao¹ZHANG San-lin² NIU Yan-fei¹ YU Xia¹GUO Zhi-kun^1*

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Abstract

Objective To study the morphological characteristics of telocytes（TCs）in neonatal rats and to verify whether TCs express the special markers of neural cells and glial cells. Methods Cardiac TCs were isolated and cultured from neonatal rats with collagenase digestion method. Immunocytochemistry, immunofluorescence and scanning electron microscopy were used to identify TCs, and to detect whether TCs had characteristics of neural and glial cells. Neonatal rat brain tissue was used as a positive control. Results Cardiac TCs were in a good state and of high purify. The immunocytology staining showed that cultured cells positively expressed vimentin and co-expressed vimentin and CD34 rather than neuron-specific enolase(NSE), glia fiber acidic protein(GFAP), OX42 and 2’3’-cyclic nucleotide 3’-phosphodiesterase (CNpase). The morphology of TCs was clear and distinguishable under a scanning electron microscope. Conclusion Cardiac TCs do not have the characteristics of neuronal and glial cells though they have some similar morphology.

Key words

Telocyte / Nerve cell / Glial cell / Primary cell culture / Neonatal rat

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GAN Li CHANG Yu-qiao ZHANG San-lin NIU Yan-fei YU Xia GUO Zhi-kun. Morphology and protein labeling of telocytes in cardiac neonatal rats[J]. Acta Anatomica Sinica. 2016, 47(5): 633-637 https://doi.org/10.16098/j.issn.0529-1356.2016.05.009

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