Effect of ginsenoside Rg1 on proliferation induced by lipopolysaccharide in BV-2 cells

YAO Yue-yi AI Qing-long CHEN Yuan-li SHANG Qun-zhu ZHANG Ru-jiang BI Xiu-mei SUN Jun* ZHONG Lian-mei*

doi:10.16098/j.issn.0529-1356.2016.05.006

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Acta Anatomica Sinica ›› 2016, Vol. 47 ›› Issue (5) : 614-619. DOI: 10.16098/j.issn.0529-1356.2016.05.006

Cell and Molecules Biology

Effect of ginsenoside Rg1 on proliferation induced by lipopolysaccharide in BV-2 cells

YAO Yue-yi^1,3 AI Qing-long²CHEN Yuan-li³ SHANG Qun-zhu² ZHANG Ru-jiang²BI Xiu-mei² SUN Jun ^3* ZHONG Lian-mei ^2*

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Abstract

Objective To investigate the possible role of ginsenoside Rg1 (Rg1) mediated in proliferating cell nuclear antigen (PCNA) and cyclin D1 of BV-2 cells stimulated by lipopolysaccharide (LPS) and to investigate the effect of Rg1 regulating proliferation in activated BV-2 cells. Methods BV-2 cells were divided into control group; LPS treatment groups with LPS stimulated for 1, 3, 6, 8, 12 hours, respectively and the Rg1 pretreatment groups in which the cells were pretreated with Rg1 (10, 20 and 50μmol/L) prior to LPS stimulate. The protein level and mRNA expression of PCNA and cyclin D1 were detected by RT-PCR and Western blotting, respectively. The expressions of cyclin D1 were detected by immunofluorescence staining. The cell cycle was detected by flow cytometry. Results The protein level and mRNA expression of PCNA and cyclin D1 in BV-2 cells were significantly increased following the treatment with LPS, which was in a time dependent manner. Compared with LPS group, addition of Rg1 inhibited the increase of PCNA and cyclin D1 in a dose-dependent manner. Conclusion Rg1 regulates the proliferation in activated BV-2 cells through inhibiting the expression of PCNA and cyclin D1.

Key words

[Key words] Rg1 / LPS / Ginsenoside Rg1 / Lipopolysaccharide / Proliferation；BV-2 cell / Proliferating cell nuclear antigen / Cyclin D1 / Flow cytometry

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YAO Yue-yi AI Qing-long CHEN Yuan-li SHANG Qun-zhu ZHANG Ru-jiang BI Xiu-mei SUN Jun ZHONG Lian-mei. Effect of ginsenoside Rg1 on proliferation induced by lipopolysaccharide in BV-2 cells[J]. Acta Anatomica Sinica. 2016, 47(5): 614-619 https://doi.org/10.16098/j.issn.0529-1356.2016.05.006

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