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Age-related changes of neural stem cells from the subventricular zone of aged mice
DONG Chuan-ming JIN Guo-hua
Acta Anatomica Sinica ›› 2016, Vol. 47 ›› Issue (5) : 577-582.
PDF(714 KB)
PDF(714 KB)
Age-related changes of neural stem cells from the subventricular zone of aged mice
Objective To investigate the biological changes of neural stem cells (NSCs) of mice from subventricular zone (SVZ) during aging. Methods NSCs were isolated, cultured and identified from 2 month (young) and 28 month (aged) old mice respectively, with 10 mice in each group. The proliferation ability of NSCs was determined by BrdU staining; cellular senescence was detected by senescence-associated-β-galactosidase (SA-β-gal) staining; ROS level was determined by ROS kit; expression of cyclin D1, p16, p19 protein in NSCs was tested by Western blotting. The thickness of SVZ between the two groups was detected by nestin/Sox2 staining.Results Positive expressions of neural stem cell marker nestin and Sox2 were identified in NSCs of the young and the aged mice. Aged NSCs exhibited a decreased percentage of BrdU positive cells, increased SA-β-gal activity, elevated intracellular reactive oxygen species level, decreased pool size in the SVZ zone, and there were significant differences (P<0.05). Additionally, at the molecular level, aged NSCs exhibited a decrease of cyclin D1 and an increase of p16 and p19.Conclusion Aged NSCs demonstrate aging changes which may play an important role in brain aging.
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