Effect of amyloid beta-peptide 25-35 neurotoxicity on cytoskeletons of&nbsp; PC12 cells

CAO Jing-jing FAN Wen-juan SHI Zhen-yu YAN Ming-chao LIU Hong-liang WANG Lai DENG Jin-bo*

doi:10.16098/j.issn.0529-1356.2016.04.06

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Acta Anatomica Sinica ›› 2016, Vol. 47 ›› Issue (4) : 469-475. DOI: 10.16098/j.issn.0529-1356.2016.04.06

Cell and Molecules Biology

Effect of amyloid beta-peptide 25-35 neurotoxicity on cytoskeletons of PC12 cells

CAO Jing-jing FAN Wen-juan SHI Zhen-yu YAN Ming-chao LIU Hong-liang WANG Lai DENG Jin-bo*

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Abstract

Objective To establish the cell model of Alzheimer’s disease (AD) and investigate the amyloid beta-peptides _25-35(Aβ _25-35) neurotoxicity to cytoskeleton.Methods PC12 cells were cultured and treated with Aβ _25-35, and cell survival was analyzed by MTT assay. Cell apoptosis was visualized with DAPI staining and TUNEL method. Immunocytochemistry and phalloidin staining were used to label cytoskeletons in PC12 cells. Results Aβ _25-35 obviously induced the PC12 cells death and lead to PC12 cells apoptosis with dose dependency (P<0.05). Aβ _25-35 gave rise to the disintegration of cytoskeletons with dose dependency (P<0.05). Conclusion PC12 cell cytoskeletons are sensitive to Aβ _25-35 neurotoxicity. The disintegration of cytoskeleton probably is the important pathological alteration in AD, and Aβ is a key molecule for AD pathogenesis.

Key words

Alzheimer’s disease / Amyloid beta-peptides 25-35 / Cytoskeleton / Terminal UTP nick end labeling / Immunohistochemistry / PC12 cell

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CAO Jing-jing FAN Wen-juan SHI Zhen-yu YAN Ming-chao LIU Hong-liang WANG Lai DENG Jin-bo. Effect of amyloid beta-peptide 25-35 neurotoxicity on cytoskeletons of PC12 cells[J]. Acta Anatomica Sinica. 2016, 47(4): 469-475 https://doi.org/10.16098/j.issn.0529-1356.2016.04.06

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