Expression changes of GZMA, GZMB, TSP-1, TLR and IL-1 five cell apoptosis pathways related genes in liver regeneration

XING Xue-kun LIU Zhen-hua LI Meng-hua XU Cun-shuan*

doi:10.16098/j.issn.0529-1356.2016.04.022

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Acta Anatomica Sinica ›› 2016, Vol. 47 ›› Issue (4) : 563-571. DOI: 10.16098/j.issn.0529-1356.2016.04.022

Bioengineering

Expression changes of GZMA, GZMB, TSP-1, TLR and IL-1 five cell apoptosis pathways related genes in liver regeneration

XING Xue-kun¹ LIU Zhen-hua¹LI Meng-hua² XU Cun-shuan^3*

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Abstract

Objective To investigate the expression changes of five cell apoptosis pathway genes, granzyme A (GZMA), granzyme B (GZMB), thrombospondin-1 (TSP-1), Toll-like receptors (TLR) and interleukin-1 (IL-1), in the process of regeneration liver (LR). Methods Rats were randomly divided into 33 groups with 6 rats in each group. Genome Rat 230 2.0 was used to detect the expression of genes in four cell apoptosis pathways in rats after partial hepatectomy (PH). Real-time PCR and Western blotting technology were used to verify the results of the chip, and the expression changes of apoptosis pathway genes in liver regeneration were analyzed by bioinformatics methods. Results Both Real-time PCR and Western blotting were in agreement with the test results of Genome Rat 230 2.0 chip; 9, 8, 24, 31 and 34 genes were associated with liver regeneration in the four cell apoptosis pathways.They were mainly expressed at the start stage of liver regeneration. The maximum number of expressed genes were in cell proliferation phase. The similarity of their expression was totally up regulation, up regulation advantage, totally down regulation, down regulation advantage, up regulation equal to down regulation of 5 categories. The expression of most genes in liver regeneration was enhanced, and the expression of few gene was decreased. Time correlation was divided into 13 groups. Analysis of the role of apoptosis pathway related genes in liver regeneration by using the gene synergy model (Et), GZMA pathway promoted apoptosis in the late stage of liver regeneration; TLR pathway promotes cell apoptosis almost in the whole liver regeneration; GZMB, TSP-1 and IL-1 pathways did not play the role of apoptosis in liver regeneration. Conclusion Two apoptosis pathways, GZMA and TSP-1, regulate apoptosis in liver regeneration.

Key words

Rat Genome 230 2.0 chip / Partial hepatectomy / Liver regeneration / Real-time PCR / Western blotting / Rat

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XING Xue-kun LIU Zhen-hua LI Meng-hua XU Cun-shuan. Expression changes of GZMA, GZMB, TSP-1, TLR and IL-1 five cell apoptosis pathways related genes in liver regeneration[J]. Acta Anatomica Sinica. 2016, 47(4): 563-571 https://doi.org/10.16098/j.issn.0529-1356.2016.04.022

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