Diacylglycerol kinase γ is involved in the neuroepithelium development of rat embryonic brain

NIU Bao-bei CUI Hui-lin* CAO Xi-mei SHI Liang MA Jin-feng QIAO Cong-jin

doi:10.16098/j.issn.0529-1356.2016.04.004

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Acta Anatomica Sinica ›› 2016, Vol. 47 ›› Issue (4) : 456-461. DOI: 10.16098/j.issn.0529-1356.2016.04.004

Neurobiology

Diacylglycerol kinase γ is involved in the neuroepithelium development of rat embryonic brain

NIU Bao-bei¹ CUI Hui-lin ^1* CAO Xi-mei¹SHI Liang¹ MA Jin-feng² QIAO Cong-jin¹

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Abstract

Objective To investigate the effects and possible mechanisms of diacylglyceral kinase γ (DGKγ) in the neuroepithelium development of the rat embryonic brain.Methods Western blotting was performed to examine the expression of DGKγ in 18 rat embryonic brains at embryonic day(E)11.5, E12.5, E14.5, E16.5 and E18.5. Five rat embryos each day from E9.5 to E18.5 were stained by immunohistochemistry and immunofluorescence to detect the distribution of DGKγ in rat embryonic brains and the colocalization of DGKγ with Ki67/choline acetyltramsferase(ChAT)/tyrosine hydroxylase(TH) in neuroepithelial cells. Results The expression of DGKγ protein increased gradually from E11.5 to E14.5, decreased significantly at E16.5 than that at E14.5, and the expression of DGKγ at E18.5 increased obviously to the level and higher than that at E14.5. At E10.5-E12.5, DGKγ was expressed from one brain vesicle to telencephalon, mesencephalon, rhombencephalon and myelencephalon except the tegmentum. At E13.5-E14.5, DGKγ was strongly expressed in hippocampus and its adjacent neocortex, pallidum, rhinencephalon and tegmentum, and then extending to all regions of brain except the neocortex. From E15.5 to before birth, the expression of DGKγ increased in neocortex and weakened in tegmentum and pons. Immunofluorescence staining showed that in DGKγ positive cells of rhinencephalon, Ki67 positive rate was higher (87%) and ChAT positive rate was lower (9%) than that of hippocampus and pallidum (all P<0.05). In tectum of E14.5 rat, 62% DGKγ positive cells showed TH positive reaction. In DGKγ positive cells of neocortex, Ki67 positive rate significantly decreased from 49% (E14.5) to 26% (E16.5), while ChAT positive rate was significantly increased from 45% (E14.5) to 70% (E16.5) (all P<0.05). The expression of DGKγ was observed in cytoplasm of neuroepithelial cells, or in membrane, or in both nucleus and cytoplasm. Conclusion The expression pattern of DGKγ in the rat embryonic brain and the subcellular distribution of DGKγ suggest that DGKγ is involved in the proliferation, migration and development of neurons.

Key words

Diacylglyceral kinase γ / Neuroepithelium / Embryonic brain / Western blotting / Immunohistochemistry / Immunofluorescence / Rat

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NIU Bao-bei CUI Hui-lin CAO Xi-mei SHI Liang MA Jin-feng QIAO Cong-jin. Diacylglycerol kinase γ is involved in the neuroepithelium development of rat embryonic brain[J]. Acta Anatomica Sinica. 2016, 47(4): 456-461 https://doi.org/10.16098/j.issn.0529-1356.2016.04.004

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