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Effect of Guizhou liquor on rat Leydig cells in the expression of steroidogenic acute regulatory protein and serum testosterone level
LIU Hao LIANG Wen-mei*
Acta Anatomica Sinica ›› 2016 ›› Issue (3) : 386-390.
Effect of Guizhou liquor on rat Leydig cells in the expression of steroidogenic acute regulatory protein and serum testosterone level
Objective To investigate effects of a Guizhou 54 degrees liquor on rat leydig cells in the expression of steroidogenic acute regulatory protein (StAR) and the change of the serum testosterone level at the different time and with different doses. Methods Sixty normal adult male SD rats were randomly divided into a normal control group(n=15) and an experimental group(n=54). Each experimental group was further subdivided into low dose group 0.8 ml/（kg·d）, middle dose group 1.6 ml/（kg·d）, high dose group 2.4 ml/（kg·d）. The rats of the experimental groups were given by gavage twice a day(11∶00 AM an 5∶00 PM)with liquor. At the end of the 4th, 8th and 12th week, the serum was collected and testicular tissue was taken. The expression of StAR was detected by immunohistochemical SABC method, image analysis and Western blotting, and level of the serum testosterone was detected by Chemiluminescence method.
Results StAR immunohistochemical positive products existed within the cytoplasm of Leydig cells; Compared with the normal control group, the average absorbance of StAR staining in the experimental groups at the 4th, 8th, and 12th week increased(P<0.05). Western blotting showed that the expression level of StAR protein in the experimental groups at the 4th, 8th and 12th week increased(P<0.05). Compared with normal control group, serum testosterone level of each experimental group did not see apparently unusual,and there was no statistically significant difference(P>0.05). Conclusion In this experiment a set of doses and schedules after gavage with the liquor enhance the StAR expression level in the rat Leydig cells enhancement.Serum testosterone level has no obvious change.
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