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Effects of secretory diarrhea on calcium activated chloride channel gene expression in the mouse
LI He-ping GUAN Yan-jing ZHA Guang-ming WANG Xin-jian WANG Yue-ying*
Acta Anatomica Sinica ›› 2016 ›› Issue (3) : 381-385.
Effects of secretory diarrhea on calcium activated chloride channel gene expression in the mouse
Objective To investigate effects of secretory diarrhea on calcium activated chloride channel（CLCAs）gene expression in the mouse intestinal tissue. Methods Twenty-four Kunming mice (12 males and 12 females) were randomly divided into three groups (n=8 in each group): control, experimental 1 hour and 8 hours groups. The control group were given a normal saline intraperitoneal injection of 0.2 ml, the experimental group were treated an by intraperitoneal injection of lipopolysaccharide (LPS，6 mg/kg) for 1hour or 8hours respectively. After injection for 1 hour or 8 hour, the mouse mental state and intestinal morphology were observed to evaluating secretory diarrhea model establishment. Real-time PCR was used to detect CLCAs gene expression in the intestine. Results The results showed that, LPS induced secretory diarrhea in mice, CLCAs genes were expressed in several intestine segments. After LPS injection, the transcriptional levels of CLCA1, CLCA2, CLCA3, CLCA4, CLCA6 gene in duodenum, jejunum, ileum and colon were up-regulated. Compared with 1 hour group, the gene transcription level significantly up-regulated in 8 hours group. After LPS injection, CLCA5 expression was not detected in duodenum, but expression of CLCA5 was significantly up-regulated in jejunum and ileum. Conclusion The different CLCAs gene expression in intestinal segments is related to secretory diarrhea caused by LPS.
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