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Comparison of microstructures of the carotid body between yaks and chaidamu yellow cattles
CHANG Lan ZHANG Shou LEI Nai-hu MA Yan-fang BAI Zhen-zhong SHEN Ming-hua GE Ri-li*
Acta Anatomica Sinica ›› 2016 ›› Issue (3) : 374-380.
Comparison of microstructures of the carotid body between yaks and chaidamu yellow cattles
Objective To compare the structural characteristics of the carotid body （CB） between yaks and chaidamu yellow cattles. Methods Health adult yaks (n=5) and chaidamu yellow cattles (n =5) were used in this study. The microstructure and ultrastructure of CB were observed by histologial, immunohistochemical SP methods and transmission electron microscopy techniques. The volume density(Vv), surface density(Sv), numerical density on area(NA) and specific surface(δ) of type Ⅰ cells(light and dark) and type Ⅱ cells and microvessels of carotid body were compared by light microscopy and microstereology between yaks and chaidamu yellow cattles. Results The CBs of yak and chaidamu yellow cattle were composed of parenchyma and mesenchyme. There were many round or oval gloubs with rich parenchymal cells. There were more type Ⅰ cells in yaks than in chaidamu yellow cattles, but there were less and smaller mitochondria and electron densecored vesicles(EDCV) of the type I cells in yaks than in chaidamu yellow cattles. The connective tissue among gloubs and interstitial cells in chaidamu yellow cattles were significantly more than yaks, but there were more rich capillaries in mesenchyme and bigger vascular lumen in yaks than in chaidamu yellow cattles. The Vv、Sv、NAof CB light cells in yaks were significantly greater than that in chaidamu yellow cattles (P<0.01),while δ of CB light cells in yaks was less than in chaidamu yellow cattles(P>0.05). The Vv、Sv of CB dark cells in yaks were significantly less than in chaidamu yellow cattles (P<0.05), and there was no significant difference of NAand δ between yaks and chaidamu yellow cattles(P>0.05). In type Ⅱ cells, there was no significant difference in Vv、Sv、NA、δ between two groups (P>0.05). The Vv、Sv、NA of the CB microvessels in yaks were obviously greater than that in chaidamu yellow cattles (P<0.01), while δ in yaks CB was significantly less than in chaidamu yellow cattle (P<0.01). Conclusion The inheret tissue cell composition and vascular net of yak CB are formed to enhance the adaptability to plateau hypoxic environment.
［1］Bu FZh, Tu DT, Cheng ZhM, et al. Light microscopic observation study of carotid bodies at human［J］. Chinese Journal of Pathophysiology, 1999, 15(3):287-293.(in Chinese)
卜凤珍,屠道同, 程仲谋,等. 高原成人颈动脉体的光镜观察和体视学研究［J］. 中国病理生理杂志,1999,15(3):287-293.
［2］Wang X, Zhang XJ, Li X, et al. Expression of interleukin-1β and its type I receptor mRNA in rat carotid body［J］. Acta Anatomica Sinica, 2006, 37(6):677-680.(in Chinese)
王曦, 张西京, 李鑫, 等. 白细胞介素-1β及其I型受体mRNA在大鼠颈动脉体中的表达［J］.解剖学报, 2006,37(6):677-680.
［3］Machiko S, Eric WK, Luis EP. Carotid chemoreceptor development in mice［J］. Resp Physiol Neurobi, 2013, 185(1):20-29.
［4］Ding YF, Li YL, Schultz HD. Role of blood flow in carotid body chemoreflex function in heart failure［J］. J Physiol, 2011, 589(1):245-258.
［5］Najafi G, Soltanalinejad F, Hasanzadeh H. A preliminary anatomical study on carotid body of Makouei sheep［J］. Vet Res Forum, 2013, 4(2):129-131.
［6］Zheng FSh. Solid Metrology of Cell Morphous ［M］. Beijing:Beijing Medical University, Peking Union Medical College United Publishing House,1990:19-86. (in Chinese)
郑富盛.细胞形态立体计量学［M］.北京:北京医科大学中国协和医科大学联合出版社,1990:19-86.
［7］Dhillon DP. The enlarged carotid body of the chronically hypoxia and hypercapnic rat: morphometric analysis［J］. Quart J Exper Physjol,1984, 69(2):301-302.
［8］Vinhaes ENG, Dolhnikoff M, Saldiva PHN. Morphological changes of carotid bodies in acute respiratory distress syndrome: a morphometric study in humans［J］. Braz J Med Biol Res, 2002, 35(10): 1119-1125.
［9］Tu DT, Bu FZh, Cheng ZhM. A morphologic study on human carotid bodies at high altitue［J］. Journal of High Altitude Medicine,1992, 2(2):20-23. (in Chinese)
屠道同,卜风珍,程仲谋.高原人的颈动脉体形态学研究［J］. 高原医学杂志,1992, 2(2):20-23.
［10］Smith DP, Heath D, Williams D, et al. The earliest histopathological response to hypobaric hypoxia in rabbits in the refugio Torino(3370 m) on Monte Bianco［J］. J Pathol, 1993, 170（4）: 485-491.
［11］Luengo AP, Granero SG, Durán R, et al. An O2-sensitive glomus cell-stem cell synapse induces carotid body growth in chronic hypoxia［J］. Cell, 2014, 156(1-2):291-303.
［12］Pardal R, Sáenz PO, Durán R, et al. Glia-like setm cells sustain physiologic neurogenesis in the adult mammalian carotid body［J］. Cell, 2007,131(2):364-377.
［13］Clarke JA, Daly MDB, Ead HW. Dimensions and volume of the carotid body in the adult cat and their relation to the specific blood flow through the organ. A histological and morphometric study［J］. Acta Anat (Basel), 1986,126(2):84-86.
［14］Yang FX, Xue DH, Zhu JG. A stereological study of the carotid bodies exposed to high-altitued hypoxid［J］. Journal of Qinghai Medical, 1993,14(3,4):145-151.(in Chinese)
杨风乡,薛大海，朱建刚.高原低氧大鼠颈动脉体的体视学研究［J］.青海医学院学报,1993, 14(3,4):145-151.
［15］Keith J，Buckler. TASK channels in arterial chemoreceptors and their role in oxygen and acid sensing［J］. Eur J Physiol,2015,467(5):1013-1025.
［16］McGregor KH, Gil J, Lahiri S. A morphometric study of the carotid body in chronically hypoxic rats［J］. J Appl Physiol Respir Environ Exerc Physiol, 1984,57(5):1430-1438.
［17］Rio RD, Mu?oz C, Arias P, et al. Chronic intermittent hypoxia-induced vascular enlargement and VEGF up regulation in the rat carotid body is not prevented by antioxidant treatment［J］. Am J Physiol Lung Cell Mol Physiol， 2011,301(5):L702-711.
［18］Raffaele DC, Veronica M, Martina MS, et al. Structural and neurochemical changes in the maturation of the carotid body［J］. Resp Physiol Neurobi, 2013, 185(1): 9-19.
［19］Donovan LM, Chai S, Gillombardo CB, et al. Ventilatory behavior and carotid body morphology of Brown Norway and Sprague Dawley rats［J］. Resp Physiol Neurobi, 2011, 178(2): 250-255.
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