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Expression and significance of synaptopodin and cytochrome C in podocytes injuries induced by palmitate acid
QI Fang ZANG Dong-yu AI Hao LI Xiao-ming*
Acta Anatomica Sinica ›› 2016 ›› Issue (3) : 337-340.
Expression and significance of synaptopodin and cytochrome C in podocytes injuries induced by palmitate acid
Objective To investigate the expression and significance of synaptopodin and cytochrome C(Cyt C) in podocyte injuries induced by palmitate acid. Methods Podocytes were cultured and randomly assigned into two groups, normal group and palimitate acid group.The two groups were cultured for 24hours, 72hours and 120hours. Cell viability of podocytes induced by palmitate acid was examined by MTT. The expressions of synaptopodin and Cyt C of podocytes were examined by immunofluorescence and western blotting. Results Compared with the normal group, cell viability of podocytes was decreased in the palimitate acid group in a time dependence manner. The immunofluorescence and Western blotting results showed that expression of Cyt C was enchanced in podocytes induced by palmitate acid. The expression of synaptopodin was reduced in podocytes induced by palmitate acid. Conclusion Palmitate acid induces that Cyt C is released and synaptopodin is abnormally expressed in podocytes, which may result in podocyte injuries eventually.
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