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Regulatory role of GADD45α in rat liver regeneration
YANG Xian-guang ZHU Lin ZHAO Wei-ming HE Chun-cui XU Cun-shuan*
Acta Anatomica Sinica ›› 2016 ›› Issue (3) : 323-329.
Regulatory role of GADD45α in rat liver regeneration
Objective To explore the role and mechanism of growth arrest and DNA damage inducible gene GADD45α during liver regeneration (LR) after partial hepatectomy (PH) in rats. Methods 114 rats were randomly divided into 19 groups, 9 groups of 2/3 liver resection, 9 control groups and 1 normal control group. The rat PH model was established, rat Genome 230 2.0 array was used to detect the gene expression profiles of the regenerated liver after PH, and Real-time PCR technique was used to verify the reliability of the microarray. Expression profile function (Ep) was used to analyze the biological processes that regulated by GADD45α, and then Ingenuity Pathway Analysis (IPA) was used to summarize the signal pathway of GADD45α regulating LR and analyze its possible mechanism. Results The microarray results showed that Gadd45α was up-regulated at 2-6 hours, 24 hours and 36-72 hours after PH. The IPA results indicated that GADD45α regulated various physiological activities which were involved in rat LR via NF-κB, p38PRAK, p53, STAT3-p21, STAT3-Bcl-2 and STAT3-cMyc paths. The results revealed that changes of physiological activity were in line with the basic process of rat LR. Conclusion GADD45α may regulate cell proliferation, cell cycle and cell survival and other physiological activities during the regeneration of liver in rat through the above pathways.
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