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Expression changes of AKT, ATM, D4-GDI and p53 cell apoptosis pathway genes in the process of rat liver regeneration
XING Xue-kun LI Meng-hua XU Cun-shuan *
Acta Anatomica Sinica ›› 2016 ›› Issue (3) : 315-322.
Expression changes of AKT, ATM, D4-GDI and p53 cell apoptosis pathway genes in the process of rat liver regeneration
Objective To investigate the expression changes of four cell apoptosis pathway genes, AKT, ATM, D4-GDI and p53 in the process of liver regeneration.
Methods The rats were randomly divided into enperiment group and control group, 6 rats in each group, half male and half female. Genome Rat 230 2.0 was used to detect the expression of genes in four cell apoptosis pathways in rats after partial hepatectomy (PH), and the biological information method was used to analyze it. Results 63, 8, 6 and 7 genes were associated with liver regeneration in the four cell apoptosis pathways.They were mainly expressed at the start stage of liver regeneration. The similarity of their expression was including totally up regulation, up regulation advantage, totally down regulation, down regulation advantage, up regulation equal to down regulation of 5 categories. The expression of most genes in liver regeneration was enhanced, and the expression of few genes was decreased. Time correlation was divided into 11 groups. Analysis of the role of apoptosis pathway related genes in liver regeneration by using the gene synergy model (Et) indicated that AKT pathway inhibited the apoptosis of cells in the whole liver regeneration; ATM, D4-GDI and p53 three cell apoptosis pathway in the promotion of cell apoptosis in the whole liver regeneration. Conclusion Four apoptosis pathways, AKT, ATM, D4-GDI and p53, are closely related to the growth, development, and liver volume control of liver regeneration.
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