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An improved glial scar model after central nervous system injury in vitro
LI Yi TAN Ling LIN Wei-wei CHEN Xue CHEN Ying PAN Jing-ying WANG Xiao-dong*
Acta Anatomica Sinica ›› 2016 ›› Issue (3) : 303-308.
An improved glial scar model after central nervous system injury in vitro
Objective To establish a scar model in vitro and observe the morphology and extracellular matrix of the scar. Methods Astrocytes and meningeal fibroblasts were cultured from the cerebral cortex in vitroand identified by glial fibrillary acidic protein (GFAP) and fibronectin(FN) immunofluorescence cell staining respectively. Astrocytes and meningeal fibroblasts were co-cultured and treated by TGF-β1 after 2 days. The group without TGF-β1 treating was used as the control. The morphology of scar was observed by GFAP and FN immunofluorescence cell staining. Immunofluorescence cell staining and Western blotting were used to examine the expression of EphB2, ephrinB2, neurocan and NG2 in the scar. Results The scar-like cell cluster was formed from astrocytes and fibroblasts. In the TGF-β1 group, the expressions of EphB2 and ephrinB2, neurocan and NG2 were significantly increased when compared with the control group (P<0.05). Conclusion A scar model after central nervous system injury has bean established by co-culturing astrocytes and fibroblasts treate by TGF-β1 in vitro.
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