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Effects of ischemia and hypoxia on DNA methylation in mouse hippocampus
LI Hong SHI Zhen-yu LI Rui-ling ZHANG Pei FU Su DENG Jin-bo* DENG Jie-xin*
Acta Anatomica Sinica ›› 2016 ›› Issue (3) : 289-296.
Effects of ischemia and hypoxia on DNA methylation in mouse hippocampus
Objective To establish a hypoxic-ischemic brain slice model, and to investigate the neuronal injuries and DNA methylations on the mouse hippocampus after hypoxic-ischemic brain damage. Methods Healthy adult C57BL / 6J mouse hippocampal slices were used to establish a HIBD model. The hypoxic-ischemic model was tested with TTC staining, and the changes of inflammatory injury and the expression of enzymes relating to DNA methylationwas were visualized by immunohistochemical and Western blotting. Results Cells’ oxidative stress and inflammatory damage of HIBD hippocampal slices were much more serious than that in the control group (P<0.01), while the level of DNA methylation was higher, compared to the control group (P<0.01). Conclusion Hypoxic-ischemic can induce the hippocampal inflammatory damages, and enhance the level of DNA methylation, suggesting that DNA methylation is involved in the process of hypoxic-ischemic tissue injury. The relative mechanism may be that HIBD can cause the DNA methylation. Conversely, DNA methylation can increase oxidative stress and hippocampal damage.
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